Team:VictoriaBC/Testing

From 2009.igem.org

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Revision as of 07:40, 20 October 2009


HOME
TESTING

Temperature Room temperature 30°C 34°C 37°C 42°C  
K235032 A expected result g g g g g This should be repressed (limited green) without lactose or IPTG independent of temperature.
K235032 A actual result G G G G G These were quite green.
K235032 B + IPTG expected G G G G G This should be induced by IPTG and produce green independent of temperature.
K235032 B +IPTG actual g g g g g These were green.
K235033 A expected / / / / / Negative control to account for microscope artifacts - there is no colour producing part at all.
K235033 A actual / / / / / This is as expected.
K235036 A expected / / R R R This should be controlled by the 32°C thermometer and red should be repressed under 32°C.
K235036 A actual / / / / / No flourescence.
K235037 A expected / / G G G This should be controlled by the 32°C thermometer and green should be repressed under 32°C.
K235037 A actual / / / / / No flourescence. 
K235005 A expected R R R R R This has no promoter but might produce limited red independent of temperature.
K235005 A actual / / / / / No flourescence. 
J04630 expected G G G G G This has no promoter but might produce limited green independent of temperature.
J04630 actual / / / / / No flourescence. 
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