Team:Victoria Australia/Notebook

- Team meeting in which we discussed ideas of how we are going to get the biological lighting system to work

- Discussed potential sponsors and other fundraising ideas

- Team meeting discussed fundraising ideas – a silent auction cocktail was an idea

- Came up with two systems to light the biological lighting system:

- Cell free systems

- Blue tongue orbivirus system

- Team meeting brainstormed ideas for the silent auction, made a list of people to invite and things to auction of.

- Discussed the advantages and disadvantages of the two systems

- Found protocols to put the fluorescent protein into the E.coli system

- Began working on the Sponsorship template

- Team meeting came to a vote to use the cell free systems for our biological lighting system and discussed using E.coli strain from cell free chassis submitted to iGEM parts registry

- Found GFP parts available through iGEM toolkit

o BBa_E0030 – Yellow Fluorescent protein (A. Victoria)

o BBa_E0020 – Cyan Fluorescent protein (A. Victoria)

o BBa_E0010 – Red Fluorescent protein (D. Striata)

o BBa_E0040 – Green Fluorescent protein (A. Victoria)

- Contacted Airline companies in regards to sponsorship

- Contacted New England Biolabs in regards to E.coli strains

- Received an email from New England Biolabs in regards of the E.coli strains- as we decided to use the protein expression strains i.e. PR1031 or ER2508

- Two students along side our supervisor went to Japan for an iGEM workshop