Team:Virginia/10 June 2009

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Overnight Results

  • Only saw growth in pSB1C3 broth
  • Have colonies on A09-1(2) plate

Today

  • Made 5ml overnight broths from 3 colonies on A09-1(2)
  • Miniprepped pSB1C3
    • 18.4 ng/ul
  • Re-transformed vector DNA in DB3.1
    • included control transformation in DH5a, should yield no colonies
  • Prepared -20 glycerol stocks of knockouts JW3469 and JW3470.
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