Team:Virginia/15 June 2009


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Overnight Results

  • Plate of A09-2 dilute (PRE BFP) had colonies
    • Still not seeing visible BFP


  • Ordered Arsenic binding peptide (ABP) as pair of oligos
    • BB prefix & suffix
    • Two repeats of CCGCG (Cys-Cys-Gly-Cys-Gly) with Lys-Gly-His linkers after each
    • Complete AA sequence: CCGCGKGHCCGCGKGH
    • Complete DNA sequence: gaattcgcggccgcttctagATGTGTTGTGGTTGCGGTAAAGGTCATTGTTGTGGTTGTGGTAAAGGTCATtactagtagcggccgctgcag
  • Getting quote for PCS in DNA 2.0's pJ express in-house expression vector.
    • with BB suffix, no BB prefix
    • Can add the prefix later with PCR, but its stop codon would interfere with expression