Team:Virginia/1 June 2009

From 2009.igem.org

(Difference between revisions)
(New page: Used the total genome extract from friday to do PCR run with ArsC primers. *used low concentration of MgCl .7 mM.)
(Today)
 
(3 intermediate revisions not shown)
Line 1: Line 1:
-
Used the total genome extract from friday to do PCR run with ArsC primers.
+
{{Template:VirginiaNotebook}}
-
*used low concentration of MgCl .7 mM.
+
 
 +
==Overnight Results==
 +
*All plates had colonies
 +
**Including negative control
 +
**BFP + B0032 assembly had few colonies
 +
 
 +
==Today==
 +
*Ran PCR of E.coli total genomic isolate with primers for ArsC
 +
**Used the total genome extract from Friday to do PCR run with ArsC primers.
 +
 
 +
*Extracted plasmid DNA from MED1, PSB1K3, BFP + B0032, and PSB1A3.*
 +
*Ran spectrophotometer on extracted plasmid DNA.*
 +
 
 +
*Digested MED1 and BFP + B0032 with X and P.*
 +
--[[User:Bsf2u|Bsf2u]] 20:45, 1 June 2009 (UTC)

Latest revision as of 20:45, 1 June 2009

Home || Back to Notebook || Upload image

Overnight Results

  • All plates had colonies
    • Including negative control
    • BFP + B0032 assembly had few colonies

Today

  • Ran PCR of E.coli total genomic isolate with primers for ArsC
    • Used the total genome extract from Friday to do PCR run with ArsC primers.
  • Extracted plasmid DNA from MED1, PSB1K3, BFP + B0032, and PSB1A3.*
  • Ran spectrophotometer on extracted plasmid DNA.*
  • Digested MED1 and BFP + B0032 with X and P.*

--Bsf2u 20:45, 1 June 2009 (UTC)