Team:Virginia/1 June 2009

From 2009.igem.org

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(Today)
(Today)
 
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==Today==
==Today==
*Ran PCR of E.coli total genomic isolate with primers for ArsC
*Ran PCR of E.coli total genomic isolate with primers for ArsC
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**Used the total genome extract from friday to do PCR run with ArsC primers.
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**Used the total genome extract from Friday to do PCR run with ArsC primers.
 +
 
 +
*Extracted plasmid DNA from MED1, PSB1K3, BFP + B0032, and PSB1A3.*
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*Ran spectrophotometer on extracted plasmid DNA.*
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*Digested MED1 and BFP + B0032 with X and P.*
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--[[User:Bsf2u|Bsf2u]] 20:45, 1 June 2009 (UTC)

Latest revision as of 20:45, 1 June 2009

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Overnight Results

  • All plates had colonies
    • Including negative control
    • BFP + B0032 assembly had few colonies

Today

  • Ran PCR of E.coli total genomic isolate with primers for ArsC
    • Used the total genome extract from Friday to do PCR run with ArsC primers.
  • Extracted plasmid DNA from MED1, PSB1K3, BFP + B0032, and PSB1A3.*
  • Ran spectrophotometer on extracted plasmid DNA.*
  • Digested MED1 and BFP + B0032 with X and P.*

--Bsf2u 20:45, 1 June 2009 (UTC)