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Team:Virginia/23 July 2009
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*PCRed HA tag onto PCS | *PCRed HA tag onto PCS | ||
*Ran gel of tagged PCS-HA | *Ran gel of tagged PCS-HA | ||
| - | **[Image:PCS-HA. | + | **[[Image:PCS-HA.JPGâ]] |
| + | **Product of PCR on PCS in attempt to add HA tag | ||
| + | **Results indicate that there was no PCR product. | ||
Latest revision as of 18:44, 27 July 2009
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Overnight Results
Today
- Ligated A09-10
- ABP-HA E,P
- pSB1C3 E,P
- Versions 1, 2, and 3 reflect different ratios of vector to insert. 1:2, 1:6, 1:1 respectively.
- Transformed A09-10 into DH5alphas
- PCRed HA tag onto PCS
- Ran gel of tagged PCS-HA
- Product of PCR on PCS in attempt to add HA tag
- Results indicate that there was no PCR product.
