Team:Virginia/3 June 2009

From 2009.igem.org

(Difference between revisions)
(Today)
m (Today)
Line 18: Line 18:
**JW3470-1 (arsC knockout)
**JW3470-1 (arsC knockout)
 +
*Set up dilutions of DH5a E.coli to get OD v cell count calibration
{| border="1"
{| border="1"
|+Cell Count of Serial 1:10 dilutions
|+Cell Count of Serial 1:10 dilutions

Revision as of 19:57, 3 June 2009

Home || Back to Notebook || Upload image

Overnight Results

Results of plating

  • psB1T3 Nothing
  • psB1C3 Small colonies
  • psB4A5 lawn
  • psB1A3 lawn
  • psB1K3 Small colonies

Today

  • Mini-Prep of Factor 7.
  • Repeat of growth curve standards.
    • k12
    • DBH5-alpha
  • Glycerol stocks (15%) for -80 freezer of
    • JW3469-1 (arsB knockout)
    • JW3470-1 (arsC knockout)
  • Set up dilutions of DH5a E.coli to get OD v cell count calibration
Cell Count of Serial 1:10 dilutions
Plate Num. Optical Density Original Concentration (X unknown) 1:10^1 1:10^2 1:10^3 1:10^4 1:10^5 1:10^6 1:10^7 1:10^8
1 0.57 X
2 0.41 .75X
3 0.27 .5X
4 0.1 .25X
5 0 .1X