Team:Warsaw/Calendar-Main/1 September 2009

From 2009.igem.org

(Difference between revisions)
 
Line 38: Line 38:
</ul>
</ul>
<br />
<br />
-
 
+
<h3><div style="text-align: center;">Cloning switch 1 regulatory parts [
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012">K177012</a>-PcI.RBS.LacI,
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177033">K177033</a>-PcI.RBS.LacI.PcI.RBS.RFP.terminator,
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177011">K177011</a>-PLacI.RBS.cI.terminator,
 +
<a href="http://partsregistry.org/Part:BBa_K177038">K177038</a>-PLacI.RBS.cI.terminator.PLacI.RBS.GFP.terminator
 +
]
 +
into two compatible low copy number plasmids of different antibiotic resistance</div></h3>
 +
<h4>Ania</h4>
 +
<br/>
 +
<p>Tasks:</p>
 +
<ul>
 +
<li>
 +
</li>
 +
</ul>
 +
<br/>
</html>
</html>
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Latest revision as of 00:36, 10 September 2009


Previous day
return to main notebook page
Previous entry
next notebook entry

 


Making of the plac-RBS-llo-intA part

Jarek


Tasks:

  • Isolation of plasmid DNA from liquid cultures.
  • Digestion of DNA samples with EcoRI and PstI endonucleases.

Cloning of the mgtc promoter into the pSB1A3 plasmid

Kamil


Tasks:

  • Bacteria transformation

Methods:

  • A fresh batch of chemocompetent bacteria was transformed with the ligation mix and incubated on agarose plates containing double dose of ampicilin

Cloning of the cro-box into the pSB1A3 plasmid

Kamil


Tasks:

  • Bacteria transformation

Methods:

  • A fresh batch of chemocompetent bacteria was transformed with the ligation mix and incubated on agarose plates containing double dose of ampicilin

Cloning switch 1 regulatory parts [ K177012-PcI.RBS.LacI, K177033-PcI.RBS.LacI.PcI.RBS.RFP.terminator, K177011-PLacI.RBS.cI.terminator, K177038-PLacI.RBS.cI.terminator.PLacI.RBS.GFP.terminator ] into two compatible low copy number plasmids of different antibiotic resistance

Ania


Tasks:


April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31