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Team:Warsaw/Calendar-Main/4 September 2009
From 2009.igem.org
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* Detailed protocol of transformation is described [https://2009.igem.org/Team:Warsaw/Calendar-Main/5_July_2009 here] | * Detailed protocol of transformation is described [https://2009.igem.org/Team:Warsaw/Calendar-Main/5_July_2009 here] | ||
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| + | <h3><div style="text-align: center;">Bistable switch 1 tests<h3> | ||
| + | <h4>Ania</h4> | ||
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| + | <p>Tasks:</p> | ||
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Revision as of 01:09, 10 September 2009
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Acquiring the internaline A gene from Listeria monocytogenes st. EDG-e genome
Jarek
Tasks:
- Electrophoretical segregation of PCR products from previous day in 0,8% agarosis gel
- Preparation of another PCR reaction with L.monocytogenes genome DNA
Results:
- There were no visible products on gel, it might happened that I accidently forget to add one of the substrates to reaction.
Assembly of endosomal detection operon
Marcin
Task 1:
- Transformation of chemocompetent E. coli TOP10 strain. Construct to transform:
Methods:
- Detailed protocol of transformation is described here
Bistable switch 1 tests
Ania
Tasks:
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April
M T W T F S S
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May
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June
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July
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August
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September
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October
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Ania
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