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Team:Warsaw/Calendar-Main/9 September 2009
From 2009.igem.org
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*Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described [http://aabiot.com/products/dna_purification/plasmid_dna/plasmid_mini/protocol_plasmid_mini.pdf here] | *Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described [http://aabiot.com/products/dna_purification/plasmid_dna/plasmid_mini/protocol_plasmid_mini.pdf here] | ||
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| + | <h3><div style="text-align: center;">Inverting PLacI regulated parts of the switch and assembling the switch on a single plasmid<h3> | ||
| + | <h4>Ania</h4> | ||
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| + | <p>Tasks:</p> | ||
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Revision as of 01:20, 10 September 2009
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Acquiring the internaline A gene from Listeria monocytogenes st. EDG-e genome
Jarek
Tasks:
- Transformation of competent E.coli strain TopF' with ligation.
- Plating ligation on LB+X-Gal+IPTG+Amp plates and leaving for incubation in 37C degree.
Assembly of fusion proteins
Marcin
Task 1 (done by Kuba):
- Prepare the bacterial cultures for isolation of following construct:
- mitochondrial signal peptide from Cox CDS on pKSII plasmid
Task 2
- isolation of previously described construct
Methods:
- Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described here
Inverting PLacI regulated parts of the switch and assembling the switch on a single plasmid
Ania
Tasks:
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April
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Ania
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