Team:Wash U/Biological Parts
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[[Image:Slide2.jpg|480px|left]] The first part of our characterization begins with the puc promoter. The puc promoter is what turns on the entire system both naturally in ''Rhodobacter sphaeroides'' and in our modified system. It is important that we have a baseline to compare the two systems so that we can determine how much efficiency is gained with the modified system given equal transcription of the puc genes. By attaching Green Fluorescent Protein (GFP) to the promoter we can quantify the rate of transcription by measuring the emittance of green light using fluorescence spectrophotometer. | [[Image:Slide2.jpg|480px|left]] The first part of our characterization begins with the puc promoter. The puc promoter is what turns on the entire system both naturally in ''Rhodobacter sphaeroides'' and in our modified system. It is important that we have a baseline to compare the two systems so that we can determine how much efficiency is gained with the modified system given equal transcription of the puc genes. By attaching Green Fluorescent Protein (GFP) to the promoter we can quantify the rate of transcription by measuring the emittance of green light using fluorescence spectrophotometer. | ||
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Revision as of 21:31, 9 July 2009