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Team:Wash U/Calendar-Home/15 July 2009
From 2009.igem.org
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-Gel purification from yesterday yielded low DNA concentrations | -Gel purification from yesterday yielded low DNA concentrations | ||
| - | -Started anew today with same digestions but only gel purifying the destination plasmids | + | |
| + | -Started anew today with same digestions (OmpC, GFP) but only gel purifying the destination plasmids | ||
| + | |||
| + | Gel layout: | ||
| + | |||
| + | 1) 100 bp ladder | ||
| + | |||
| + | 2) OmpC + vector (2187 bp) | ||
| + | |||
| + | 3) OmpC + vector | ||
| + | |||
| + | 4) GFP (876 bp) | ||
| + | |||
| + | 5) GFP | ||
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| + | 6) Destination plasmid, pSB1K3 (2206 bp) | ||
| + | |||
| + | 7) pSB1K3 | ||
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| + | 8) 1 kb ladder | ||
Revision as of 17:30, 15 July 2009
