Team:Wash U/Calendar-Home/15 July 2009

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(→Wednesday July 15, 2009)
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8) 1 kb ladder
8) 1 kb ladder
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Ligation reactions:
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1: 6 microliters OmpC, 8 microliters GFP, 4 microliters pSB1K3, 2 microliters buffer, 1 microliter enzyme
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2: 4 microliters OmpC, 6 microliters GFP, 4 microliters pSB1K3, 2 microliters buffer, 1 microliter enzyme, 3 microliters water

Revision as of 20:51, 15 July 2009


Wednesday July 15, 2009

-Gel purification from yesterday yielded low DNA concentrations

-Started anew today with same digestions (OmpC, GFP) but only gel purifying the destination plasmids

Gel layout:

1) 100 bp ladder

2) OmpC + vector (2187 bp)

3) OmpC + vector

4) GFP (876 bp)

5) GFP

6) Destination plasmid, pSB1K3 (2206 bp)

7) pSB1K3

8) 1 kb ladder

Ligation reactions:

1: 6 microliters OmpC, 8 microliters GFP, 4 microliters pSB1K3, 2 microliters buffer, 1 microliter enzyme

2: 4 microliters OmpC, 6 microliters GFP, 4 microliters pSB1K3, 2 microliters buffer, 1 microliter enzyme, 3 microliters water


-Team meeting #6



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