Team:Wash U/Calendar-Home/25 June 2009

From 2009.igem.org

(Difference between revisions)
(Wednesday June 24, 2009)
Line 10: Line 10:
-Minipreps of biobrick plasmid DNA (cultures from Th. 6/18)
-Minipreps of biobrick plasmid DNA (cultures from Th. 6/18)
-
-Overnight PCR with miniprepped DNA
+
-Overnight PCR with miniprepped DNA, to check for inserts
DNA nanospec concentrations (ng/microliter):
DNA nanospec concentrations (ng/microliter):

Revision as of 21:09, 25 June 2009

Wednesday June 24, 2009

-Restreaked sphaeroides plates and plated from liquid cultures. We are working on getting rid of contamination on the earlier plates and in cultures.

-Waiting on supplies from Sigma Aldrich!

-Working on our webpage.... We have viewers from 4 continents!

-Minipreps of biobrick plasmid DNA (cultures from Th. 6/18)

-Overnight PCR with miniprepped DNA, to check for inserts

DNA nanospec concentrations (ng/microliter):

1) 22.2

2) 51.4

3) 135

4) 75.9

5) 81.4

6) 31.1

7) 49.6

8) 35.9

9) 62.9

10) 52.2

11) 134

12) 45.5



PCR (1) labels

PCR, Miniprep, Part #, Conc(ng/microliter), Plasmid, Plate

(A), 1, BBa_I15010, 22.2, pSB2K3, LBK50

(B), 2, BBa_R0082, 51.4, pSB1A2, LBA50

(C), 4, BBa_B0015, 75.9, pSB1AK3, LBA50

(D), 5, BBa_J04450, 81.4, pSB1K3, LBK50

(E), 7, BBa_B0034, 49.6, pSB1A2, LBA50

(F), 9, BBa_E0240, 62.9, pSB1A2, LBA50

(G), 10, BBa_J13002, 52.2, pSB1A2, LBA50

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