Team:Wash U/Protocol

From 2009.igem.org

(Difference between revisions)
Line 22: Line 22:
* 2.5mM KCl (0.186g)
* 2.5mM KCl (0.186g)
* ddH2O to 1L
* ddH2O to 1L
-
'''Super Optimal Broth with Catabolite repression (SOC)'''
+
'''Super Optimal Broth with Catabolite repression (SOC)'''<br>
In addition to all of the SOB components,
In addition to all of the SOB components,
* 10mM MgCl2 (0.952g)
* 10mM MgCl2 (0.952g)

Revision as of 17:54, 29 June 2009

Procedures

Polymerase Chain Reaction (PCR)


Recipes

Lysogeny Broth (LB) Media

  • 10g Tryptone
  • 5g Yeast Extract
  • 10g NaCl
  • 15g Agar
  • up to 1 Liter H20

Super Optimal Broth (SOB)

  • 2% w/v bacto-tryptone (20g)
  • 0.5% w/v bacto-yeast extract (5g)
  • 8.56mM NaCl (0.5g) or 10mM NaCl (0.584g)
  • 2.5mM KCl (0.186g)
  • ddH2O to 1L

Super Optimal Broth with Catabolite repression (SOC)
In addition to all of the SOB components,

  • 10mM MgCl2 (0.952g)
  • 20mM glucose (3.603g)

Retrieved from "http://2009.igem.org/Team:Wash_U/Protocol"