Team:Wash U/Protocol
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:1. Begin BioBrick Assembly with three separate plasmids, an upstream part, a down stream part, and a destination plasmid. It is important that the destination plasmid contain the toxic gene ccdB in the BioBrick cloning site and a different antibiotic resistance to the upstream and downstream parts.<br> | :1. Begin BioBrick Assembly with three separate plasmids, an upstream part, a down stream part, and a destination plasmid. It is important that the destination plasmid contain the toxic gene ccdB in the BioBrick cloning site and a different antibiotic resistance to the upstream and downstream parts.<br> | ||
:2. Digest each part with the proper restriction enzymes to isolate the upstream and downstream parts, and to remove the BioBrick from the destination plasmid.<br> | :2. Digest each part with the proper restriction enzymes to isolate the upstream and downstream parts, and to remove the BioBrick from the destination plasmid.<br> | ||
- | :3. | + | :3. Perform a [[Ligation]] on the products. The result will be the upstream part connected to the downstream part in the destination plasmid. This new composite part can then be used to transform competent cells.<br> |
:To view the full Biobrick Assembly manual, click [http://ginkgobioworks.com/support/ here]. | :To view the full Biobrick Assembly manual, click [http://ginkgobioworks.com/support/ here]. | ||
Revision as of 17:05, 30 June 2009