Team:Wash U/Protocol
From 2009.igem.org
Line 93: | Line 93: | ||
=='''Ligation'''== | =='''Ligation'''== | ||
<font size="2"> | <font size="2"> | ||
+ | Ligation formally joins two or more pieces of DNA together that are already annealed. Note: this procedure requires the products of a successful digestion. | ||
:General description | :General description | ||
'''Materials''' | '''Materials''' | ||
:List of Materials | :List of Materials | ||
'''Procedures''' | '''Procedures''' | ||
- | |||
# Thaw the 10X T4 DNA Ligase Reaction Buffer and mix to dissolve the precipitate. | # Thaw the 10X T4 DNA Ligase Reaction Buffer and mix to dissolve the precipitate. | ||
# Add 11 uL of dH2O to a 200 uL PCR tube. Then add 2 uL of each of the digestion products (upstream, downstream and destination) together into this new tube. | # Add 11 uL of dH2O to a 200 uL PCR tube. Then add 2 uL of each of the digestion products (upstream, downstream and destination) together into this new tube. |
Revision as of 20:07, 8 July 2009