Team:Wash U/Protocol
From 2009.igem.org
(→Procedures) |
(→Gel Electrophoresis) |
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:Gel Electrophoresis is a technique used to separate out DNA according to its length. DNA is loaded into a well, or hole in an agarose gel, and is then pulled through the gel via electric current (DNA has a negative charge). Smaller DNA fragments travel faster through the gel matrix while larger fragments travel slower. To better determine the length of DNA a ladder is often run parallel to an unknown piece of DNA. The ladder consists of several pieces of DNA of known length which serve as a reference point to the unknown fragment. Note that dyes are important in this procedure since DNA alone is invisible. Specific Dyes are needed to adhered to and travel with the DNA through the gel. | :Gel Electrophoresis is a technique used to separate out DNA according to its length. DNA is loaded into a well, or hole in an agarose gel, and is then pulled through the gel via electric current (DNA has a negative charge). Smaller DNA fragments travel faster through the gel matrix while larger fragments travel slower. To better determine the length of DNA a ladder is often run parallel to an unknown piece of DNA. The ladder consists of several pieces of DNA of known length which serve as a reference point to the unknown fragment. Note that dyes are important in this procedure since DNA alone is invisible. Specific Dyes are needed to adhered to and travel with the DNA through the gel. | ||
'''Materials''' | '''Materials''' | ||
- | + | *Agarose | |
+ | *1X TAE Buffer | ||
+ | *Ethidium Bromide | ||
+ | *DNA Ladder | ||
+ | *DNA Samples | ||
+ | *Gel Pouring Dock | ||
+ | *Gel Tray | ||
+ | *Gel Box | ||
+ | *Power Source | ||
'''Procedures''' | '''Procedures''' | ||
:text | :text |
Revision as of 21:25, 9 July 2009