Team:Washington-Software

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!align="center"; style="border: #6b0c6a inset 3px;" |[[Team:Washington-Software|<font color="gold">Home</font>]]
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<h2> Abstract </h2>
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<h4>'''BioBrick-A-Bot: Lego Robot for Automated BioBrick DNA Assembly'''</h4>
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[[Image:Robot Close Up.jpg|thumb|right|'''BioBrick-A-Bot''']]
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<h1> Abstract </h1>
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<h4>'''LegoRoboBricks for Automated BioBrick Assembly'''</h4>
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Commercial Liquid Handling Systems are extremely expensive, and are typically beyond the reach of the average molecular biologist interested in performing high throughput methods.  To address this problem, we design and implement a liquid handling system built from commonly accessible Legos. Our goal is the automation of BioBrick assembly on a platform that can itself be easily replicated and we demonstrate a proof-of-principle for this system by transferring colored dye solutions on a 96-well plate. We introduce a new concept called LegoRoboBrick.  The liquid handling system is build from 3 new LegoRoboBrick modular components: ALPHA (Automated Lego Pipette Head Assembly), BETA (BioBrick Environmental Testing Apparatus), and PHI (Pneumatic Handling Interface).  We will demonstrate that the same BioBrick assembly software can run on multiple plug-and-play LegoRoboBrick instances with different physical dimensions and geometric configurations. The modular design of LegoRoboBricks allows easy extension of new laboratory functionalities in the future.
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[[Image:Robot Close Up.jpg|230px|right]]
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<h5>Commercial Liquid Handling Systems are extremely expensive, and are typically beyond the reach of the average molecular biologist interested in performing high throughput methods.  To address this problem, we design and implement a liquid handling system built from commonly accessible Legos. We demonstrate a proof-of-principle use for this system to perform BioBrick assembly by transferring colored dye solutions on a 96-well plate.
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<h2> Project Goals </h2>
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#Low Cost – Robot cost significantly lower than $10,000, the price of a commercial liquid handling robot. (Actual cost of BioBrick-a-Bot prototype: ~$700)
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#Hardware Platform that is easily accessible.
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#Hardware Design that is easily replicable by other iGEM teams.
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#Software Design  that is robust, plug and play. Can swap modules from other iGEM teams.
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#Design that is easily extensible, to allow future collaboration with other iGEM teams.
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[''Raik suggestion:'' Our goal is the automation of BioBrick assembly on a Lego platform that can itself be easily replicated (and we...) We demonstrate a proof-of-principle use for this system by transferring colored dye solutions on a 96-well plate.]
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<h2> The Vision </h2>
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We introduce a new concept called LegoRoboBrick. The liquid handling system is build from 3 new LegoRoboBrick modular components: ALPHA (Automated Lego Pipette Head Assembly), BETA (BioBrick Environmental Testing Apparatus), and PHI (Pneumatic Handling Interface). We will demonstrate that the same BioBrick assembly software can run on multiple plug-and-play LegoRoboBrick instances with different physical dimensions and geometric configurations. The modular design of LegoRoboBricks allows easy extension of new laboratory functionalities in the future.</h5>
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[[Image:LegoRoboBrick.jpg|315px|left]][[Image:LegoRoboBrick2.jpg|315px|left]][[Image:LegoRoboBricks.jpg|313px|right]]
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<h1> Project Goals </h1>
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<h2> Acknowledgements </h2>
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<h5>
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*Implement a simple and cheap way to handle liquids in normal genome lab operations(portable genomic science lab)
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*Only uses lego mindstorm bricks
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*Document entire process so it can easily be replicated </h5>
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==Check list==
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Our iGEM project is sponsored by the [http://depts.washington.edu/bioe/ BioEngineering Department] at the [http://www.washington.edu/ University of Washington]
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Home: the whole picture of the robot, abstract, project goals
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[[Image:bioelogo.jpg]]
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[[Image:uw_logo.jpg]]
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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<!--- The Mission, Experiments --->
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Latest revision as of 02:25, 22 October 2009

WashingtonColorSeal-21-clip.gif Home Team Project Modeling Notebook Challenges Miscellaneous

Contents

Abstract

BioBrick-A-Bot: Lego Robot for Automated BioBrick DNA Assembly

BioBrick-A-Bot

Commercial Liquid Handling Systems are extremely expensive, and are typically beyond the reach of the average molecular biologist interested in performing high throughput methods. To address this problem, we design and implement a liquid handling system built from commonly accessible Legos. Our goal is the automation of BioBrick assembly on a platform that can itself be easily replicated and we demonstrate a proof-of-principle for this system by transferring colored dye solutions on a 96-well plate. We introduce a new concept called LegoRoboBrick. The liquid handling system is build from 3 new LegoRoboBrick modular components: ALPHA (Automated Lego Pipette Head Assembly), BETA (BioBrick Environmental Testing Apparatus), and PHI (Pneumatic Handling Interface). We will demonstrate that the same BioBrick assembly software can run on multiple plug-and-play LegoRoboBrick instances with different physical dimensions and geometric configurations. The modular design of LegoRoboBricks allows easy extension of new laboratory functionalities in the future.

Project Goals

  1. Low Cost – Robot cost significantly lower than $10,000, the price of a commercial liquid handling robot. (Actual cost of BioBrick-a-Bot prototype: ~$700)
  2. Hardware Platform that is easily accessible.
  3. Hardware Design that is easily replicable by other iGEM teams.
  4. Software Design that is robust, plug and play. Can swap modules from other iGEM teams.
  5. Design that is easily extensible, to allow future collaboration with other iGEM teams.

The Vision

LegoRoboBrick.jpg
LegoRoboBrick2.jpg
LegoRoboBricks.jpg

Acknowledgements

Our iGEM project is sponsored by the BioEngineering Department at the University of Washington

Bioelogo.jpg

Uw logo.jpg