Team:Washington-Software

From 2009.igem.org

Revision as of 23:59, 4 October 2009

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)	Your team picture
	Team Example

(Or you can choose different headings. But you must have a team page, a project page, and a notebook page.)

Abstract

RoboLabBricks for Automated BioBrick Assembly

Commercial Liquid Handling Systems are extremely expensive, and are typically beyond the reach of the average molecular biologist interested in performing high throughput methods. To address this problem, our project consists of the design and implementation of an automated laboratory and demonstrate its use for performing high throughput BioBrick assembly. This low cost solution is built using commonly accessible LEGO bricks. We demonstrate a proof-of-principle use for BioBrick assembly by transferring colored dye solutions on a 96-well plate.

We introduce a new concept called RoboLabBricks. Currently the laboratory is built using three RoboLabBrick components: ALPHA (Automated Lego Pipette Head Assembly), BETA(Biobrick Enviormental Testing Apparatus), and PHI(Pneumatic Handling Intergace). We will demonstrate that the same BioBrick assembly software can run on multiple plug-and-play RoboLabBrick instances with different physical dimensions and geometric configurations. The modular design of RoboLabBricks allows easy extension of new laboratory functionalities in the future.

Project Goals

• Implement a simple and cheap way to handle liquids in normal genome lab operations(portable genomic science lab)
• Only uses lego mindstorm bricks
• Document entire process so it can easily be replicated

Project Summary

Hardware

• Lego Bricks
  • Commonly accessible industry standard

Firmware

• RobotC
  • Made in CMU Robotics Academy
  • Enables floating point precision

Software

• ALPHA module
  • Precise reverse triangulation using Rotational Matrix
  • Controller of Master-Slave Synchronization
  • Accurately positions pipette head
• PHI module
  • Pneumatic control to suck and dispense fluid
  • Compression pump to "air-clean" system

Mathematical Modeling

Alpha

Problem

Given the following construction and point p, or (x,y,z) find the angles θ1, θ2, and θ3.

Note that positive z is the down direction.

Constants

• TR
  • Top radius
• BR
  • Bottom radius
• L
  • Linkage
• CA
  • Control Arm
• φ1 and φ2
  • Two angles

Construction

From the top to the bottom:

• A circle centered at the origin with radius TR, named O
• Make three lines A1, A2, and A3 such that:
  • The A lines are perpendicular to a tangent of O and a radius of O,
  • The angle between the radii of A1 and A2 is φ1, and
  • The angle between the radii of A2 and A3 is φ2.
• Make a circle centered at point p with radius BR, named P
• Find three points P1, P2, and P3 such that:
  • They are on the circumfurence of P,
  • The angle between the radius which touches A1 and the radius that touches A2 is φ1,
  • The angle between the radius which touches A2 and the radius that touches A3 is φ2,
  • The ray from the center of P to Px is parallel to the ray from the center of O to the point which is on Ax and O 's circumference, for all x.
• Construct three line segments CA1, CA2, and CA3 such that:
  • CAx is in the same plane as Ax, for all x, and
  • The angle between CAx and Ax is θx, for all x.
• The distance between Px and the end point of CAx that is not on Ax is L, for all x.

RoboLabBrick Modules

Just like BioBricks are genes that can be connected to make a product, RoboLabBricks are robot modules that can be connected to make a Genomics Lab System.

Module ALPHA

ALPHA stands for Automatic Lego Pipet Head Assembly.

• Created 8/21/2009
• Consists of 3 double-jointed arms.
  • One joint is connected to the motor, and is controlled entirely by the motor.
  • The other joint moves in a sphere, and is loose. The end of this attaches to the platform which holds the pipet tip.
• Videos
  • Robot in Action
    • This video shows that the module has high accuracy and precision. The stand is module Beta.
  • Two Robots
    • This video shows that the same code can be used for other versions of ALPHA. The only difference is 6 physical constants:
      1. Top Offset
      2. Bottom Offset
      3. Control Arm Length
      4. Linkage Arm Length
      5. Inter-arm Angle
      6. Gear Ratio
  • Old Video

Module BETA

BETA stands for Biobrick Enviorment Testing Apparatus.

• Consists of a telescoping frame, and a big lego plate.
  • The telescoping frame is used for holding ALPHAs and PHIs.
  • the big lego plate is where you put the 96-well plates and petri dishes.

Module PHI

PHI stands for Pneumatics Handling Interface.

• PHI is the pipette. It consists of three motors.
  • Motor A.
    • This motor controls the flow of the air. If you look at it from the side when the switch is visible:
      • When the switch is to the left, there is a direct flow from the pipet head to the air. This makes it possible to use the second motor (motor B) to suck without sucking any liquid, and enabling it to blow extra air out.
      • When the switch is in the middle, there is no connection.
      • When the switch is to the right, the pressure built up in the air tank is released into the pipette head.
  • Motor B.
    • This motor is connected to a piston, so it can suck and dispense liquid.
  • Motor C.
    • This motor is connected to to compressors compressing air in the air tank. It runs for 7 seconds once the air is released.
• Videos
  • Phi in action
    • This video shows Phi running by itself.

Past Robots

Past Robots

• Recent changes
• What links here
• Related changes
• Special pages
• My preferences

• Printable version
• Permanent link
• Privacy policy
• Disclaimers