Team:Washington/Future

From 2009.igem.org

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===Overview===
*Target Construct
*Target Construct
# Attempt to add additional proteins into the vector and test for functionality
# Attempt to add additional proteins into the vector and test for functionality
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# Test designed proteins from FoldIt
# Test designed proteins from FoldIt
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===Current Focus===
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====New Display Vector====
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====Fold-It====
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Revision as of 19:50, 18 October 2009

Uw title logo.png

Overview

  • Target Construct
  1. Attempt to add additional proteins into the vector and test for functionality
  2. Vary linker lengths
  3. Make a simpler version for trouble shooting the secretion system: [6x-His]-[NheI]-[prtB]
  4. Transfer to a vector with the same origin and resistance as described in the original secretion system (pBR322+Carb).
  5. Add a lacI into the expression and target vectors so repression is hard-coded into the vector and expression can be induced regardless of the cell line.


  • Secretion System
  1. Transfer to a Chlor resistance, p15A origin vector as used in the original papers
  2. Add original upstream DNA (50bp) before the native RBS to ensure proper function
  3. Add an arabanose inducible promoter for better control over secretion system activation
  4. Combine with target vector so entire secretion system is contained in one plasmid.


  • Display System
  1. Test additional proteins in the new custom display construct (GFP, OpdA, etc)
  2. Test designed proteins from FoldIt

Current Focus

New Display Vector

Fold-It