Team:Washington/Notebook

From 2009.igem.org

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{{Template:Team:Washington/Templates/Header}}
{{Template:Team:Washington/Templates/Header}}
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==== Protocols ====
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= '''Protocols''' =
[[Team:Washington/Notebook/protein_gel|Protein Gel]]
[[Team:Washington/Notebook/protein_gel|Protein Gel]]
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[[Team:Washington/Notebook/Microscope|Microscope]]
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[[Team:Washington/Notebook/Microscope|Microscopy]]
[[Team:Washington/Notebook/Flow_Cytometry|Flow Cytometry]]
[[Team:Washington/Notebook/Flow_Cytometry|Flow Cytometry]]
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[[Team:Washington/Notebook/colony_PCR|Colony PCR]]
[[Team:Washington/Notebook/colony_PCR|Colony PCR]]
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[[Team:Washington/Notebook/NheI_PstI|Gene Assembly using the NheI and PstI sites]]
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[[Team:Washington/Notebook/NheI_PstI|BioBrick Assembly using the NheI and PstI sites]]
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[[Team:Washington/Notebook/NheI|BioBrick Assembly using the NheI site]]
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[[Team:Washington/Notebook/SOEingPCR|SOEing PCR]]
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[[Team:Washington/Notebook/IMAC_protocol|Traditional Protein Purification (IMAC)]]
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[[Team:Washington/Notebook/Standard_curve|Generating a Standard curve for GFP concentration]]
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<br>
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= '''Project Time Line''' =
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[[Team:Washington/Notebook/NheI|Gene Assembly using the NheI site]]
 
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==== Time Line of Project ====
 
*Winter Quarter
*Winter Quarter
**Introduction to iGEM  
**Introduction to iGEM  
**Synthetic Biology Seminar
**Synthetic Biology Seminar
**Plan for project ideas
**Plan for project ideas
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*Spring Quarter
*Spring Quarter
**Narrow down potential projects  
**Narrow down potential projects  
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**Obtained Funding
**Obtained Funding
**Stock Lab
**Stock Lab
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*June
*June
**Sequence genes
**Sequence genes
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**Test target proteins for solubility and expression
**Test target proteins for solubility and expression
**Start assembly of secretion genes
**Start assembly of secretion genes
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*July
*July
**Develop and perform assays for testing legacy surface display bio-bricks  
**Develop and perform assays for testing legacy surface display bio-bricks  
**Transfer prtDEF contig from secretion system into low copy plasmid
**Transfer prtDEF contig from secretion system into low copy plasmid
**Test target proteins for functionality
**Test target proteins for functionality
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*August
*August
**Finish assembly of secretion system
**Finish assembly of secretion system
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**Test for secretion of target protein
**Test for secretion of target protein
**Start design of new display construct
**Start design of new display construct
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*September
*September
**Switch secretion system into new cell line, make cells competent
**Switch secretion system into new cell line, make cells competent
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**Start t-shirt design  
**Start t-shirt design  
**Insert streptavidin into new display vector
**Insert streptavidin into new display vector
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*October
*October
**Fine tune secretion assay, adjust controls
**Fine tune secretion assay, adjust controls
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**Characterize target bio-bricks
**Characterize target bio-bricks
**Prepare for Jamboree
**Prepare for Jamboree
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{{Template:Team:Washington/Templates/Footer}}

Latest revision as of 20:20, 20 October 2009

Uw title logo.png

Protocols

Protein Gel

Microscopy

Flow Cytometry

Supernatant Protein Purification, 50mL

Ni-column Set up

Supernatant Protein Purification, 2mL

Gene Synthesis

Colony PCR

BioBrick Assembly using the NheI and PstI sites

BioBrick Assembly using the NheI site

SOEing PCR

Traditional Protein Purification (IMAC)

Generating a Standard curve for GFP concentration


Project Time Line

  • Winter Quarter
    • Introduction to iGEM
    • Synthetic Biology Seminar
    • Plan for project ideas


  • Spring Quarter
    • Narrow down potential projects
    • Choose Project
    • Order oligos and start synthesizing genes
    • Obtained Funding
    • Stock Lab


  • June
    • Sequence genes
    • Preliminary binding assays for biotinylated fluorophore
    • Introduction to Fold-it as a tool for protein design
    • Test target proteins for solubility and expression
    • Start assembly of secretion genes


  • July
    • Develop and perform assays for testing legacy surface display bio-bricks
    • Transfer prtDEF contig from secretion system into low copy plasmid
    • Test target proteins for functionality


  • August
    • Finish assembly of secretion system
    • Start cell lines containing various forms of secretion system, make competent
    • Transform competent cells containing secretion system with target vector
    • Test for secretion of target protein
    • Start design of new display construct


  • September
    • Switch secretion system into new cell line, make cells competent
    • Transform with target vector
    • Test for secretion
    • Start Presentation
    • Start t-shirt design
    • Insert streptavidin into new display vector


  • October
    • Fine tune secretion assay, adjust controls
    • Finalize characterization of legacy parts
    • Practice presentation
    • Characterize target bio-bricks
    • Prepare for Jamboree