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Team:Washington/Notebook
From 2009.igem.org
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| - | + | ==== Time Line of Project ==== | |
| - | + | *Winter Quarter | |
| - | + | **Introduction to iGEM | |
| + | **Synthetic Biology Seminar | ||
| + | **Plan for project ideas | ||
| + | *Spring Quarter | ||
| + | **Narrow down potential projects | ||
| + | **Choose Project | ||
| + | **Order oligos and start synthesizing genes | ||
| + | **Obtained Funding | ||
| + | **Stock Lab | ||
| + | *June | ||
| + | **Sequence genes | ||
| + | **Preliminary binding assays for biotinylated fluorophore | ||
| + | **Introduction to Fold-it as a tool for protein design | ||
| + | **Test target proteins for solubility and expression | ||
| + | **Start assembly of secretion genes | ||
| + | *July | ||
| + | **Develop and perform assays for testing legacy surface display bio-bricks | ||
| + | **Transfer prtDEF contig from secretion system into low copy plasmid | ||
| + | **Test target proteins for functionality | ||
| + | *August | ||
| + | **Finish assembly of secretion system | ||
| + | **Start cell lines containing various forms of secretion system, make competent | ||
| + | **Transform competent cells containing secretion system with target vector | ||
| + | **Test for secretion of target protein | ||
| + | **Start design of new display construt | ||
Revision as of 04:52, 15 October 2009
Protocols
Supernatant Protein Purification, 50mL
Supernatant Protein Purification, 2mL
Gene Assembly using the NheI and PstI sites
Gene Assembly using the NheI site
Time Line of Project
- Winter Quarter
- Introduction to iGEM
- Synthetic Biology Seminar
- Plan for project ideas
- Spring Quarter
- Narrow down potential projects
- Choose Project
- Order oligos and start synthesizing genes
- Obtained Funding
- Stock Lab
- June
- Sequence genes
- Preliminary binding assays for biotinylated fluorophore
- Introduction to Fold-it as a tool for protein design
- Test target proteins for solubility and expression
- Start assembly of secretion genes
- July
- Develop and perform assays for testing legacy surface display bio-bricks
- Transfer prtDEF contig from secretion system into low copy plasmid
- Test target proteins for functionality
- August
- Finish assembly of secretion system
- Start cell lines containing various forms of secretion system, make competent
- Transform competent cells containing secretion system with target vector
- Test for secretion of target protein
- Start design of new display construt
