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Team:Washington/Notebook
From 2009.igem.org
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**Transform competent cells containing secretion system with target vector | **Transform competent cells containing secretion system with target vector | ||
**Test for secretion of target protein | **Test for secretion of target protein | ||
| - | **Start design of new display | + | **Start design of new display construct |
| + | *September | ||
| + | **Switch secretion system into new cell line, make cells competent | ||
| + | **Transform with target vector | ||
| + | **Test for secretion | ||
| + | **Start Presentation | ||
| + | **Start t-shirt design | ||
| + | **Insert streptavidin into new display vector | ||
| + | *October | ||
| + | **Fine tune secretion assay, adjust controls | ||
| + | **Finalize characterization of legacy parts | ||
| + | **Practice presentation | ||
| + | **Characterize target bio-bricks | ||
| + | **Prepare for Jamboree | ||
Revision as of 04:59, 15 October 2009
Protocols
Supernatant Protein Purification, 50mL
Supernatant Protein Purification, 2mL
Gene Assembly using the NheI and PstI sites
Gene Assembly using the NheI site
Time Line of Project
- Winter Quarter
- Introduction to iGEM
- Synthetic Biology Seminar
- Plan for project ideas
- Spring Quarter
- Narrow down potential projects
- Choose Project
- Order oligos and start synthesizing genes
- Obtained Funding
- Stock Lab
- June
- Sequence genes
- Preliminary binding assays for biotinylated fluorophore
- Introduction to Fold-it as a tool for protein design
- Test target proteins for solubility and expression
- Start assembly of secretion genes
- July
- Develop and perform assays for testing legacy surface display bio-bricks
- Transfer prtDEF contig from secretion system into low copy plasmid
- Test target proteins for functionality
- August
- Finish assembly of secretion system
- Start cell lines containing various forms of secretion system, make competent
- Transform competent cells containing secretion system with target vector
- Test for secretion of target protein
- Start design of new display construct
- September
- Switch secretion system into new cell line, make cells competent
- Transform with target vector
- Test for secretion
- Start Presentation
- Start t-shirt design
- Insert streptavidin into new display vector
- October
- Fine tune secretion assay, adjust controls
- Finalize characterization of legacy parts
- Practice presentation
- Characterize target bio-bricks
- Prepare for Jamboree
