http://2009.igem.org/wiki/index.php?title=Team:Washington/Notebook/Flow_Cytometry&feed=atom&action=historyTeam:Washington/Notebook/Flow Cytometry - Revision history2024-03-28T15:01:25ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:Washington/Notebook/Flow_Cytometry&diff=125374&oldid=prevRobere: /* Flow Protocol Reference */2009-10-20T07:13:43Z<p><span class="autocomment">Flow Protocol Reference</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Record cytometry data again with reduced fluorescence background</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Record cytometry data again with reduced fluorescence background</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==== Flow Protocol <del class="diffchange diffchange-inline">Reference </del>====</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==== Flow Protocol <ins class="diffchange diffchange-inline">References </ins>====</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Isolating and engineering human antibodies using yeast surface display. Chao G et al. [http://www.ncbi.nlm.nih.gov/pubmed/17406305 Nat Protoc. 2006;1(2):755-68]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Isolating and engineering human antibodies using yeast surface display. Chao G et al. [http://www.ncbi.nlm.nih.gov/pubmed/17406305 Nat Protoc. 2006;1(2):755-68]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#Cell division in ''Escherichia coli'' cultures monitored at single cell resolution. Roostalu J et al. [http://www.biomedcentral.com/1471-2180/8/68 BMC Microbiology 2008, 8:68; doi:10.1186/1471-2180-8-68]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Team:Washington/Templates/Footer}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Team:Washington/Templates/Footer}}</div></td></tr>
</table>Roberehttp://2009.igem.org/wiki/index.php?title=Team:Washington/Notebook/Flow_Cytometry&diff=125365&oldid=prevRobere: /* Flow Cytometry */2009-10-20T07:10:58Z<p><span class="autocomment">Flow Cytometry</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Record cytometry data again with reduced fluorescence background</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Record cytometry data again with reduced fluorescence background</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==== Flow Protocol Reference ====</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#Isolating and engineering human antibodies using yeast surface display. Chao G et al. [http://www.ncbi.nlm.nih.gov/pubmed/17406305 Nat Protoc. 2006;1(2):755-68]</ins></div></td></tr>
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</table>Roberehttp://2009.igem.org/wiki/index.php?title=Team:Washington/Notebook/Flow_Cytometry&diff=125270&oldid=prevRobere: /* Flow Cytometry */2009-10-20T07:00:22Z<p><span class="autocomment">Flow Cytometry</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Flow Cytometry ====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Flow Cytometry ====</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Set up overnights of parts J36848-J36851 in terrific broth (TB). Let grow overnight at 37 &deg;C.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Set up overnights of parts J36848-J36851 in terrific broth (TB). Let grow overnight at 37&deg;C.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Dilute 20 &mu;L overnight into 1 mL TB and grow at 37 &deg;C until OD 0.3 to OD 0.8.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Dilute 20 &mu;L overnight into 1 mL TB and grow at 37&deg;C until OD 0.3 to OD 0.8.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Add IPTG to 1 mM concentration and let grow for minimum of four hours at room temperature (~23 &deg;C).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Add IPTG to 1 mM concentration and let grow for minimum of four hours at room temperature (~23&deg;C).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Record OD 600 and add equivalent of 1 &mu;L of OD 2.0 cells to 1mL PBS with 1 mg/mL BSA and specified flourophore concentration (0-100 nM) and allow time to bind (30 minutes to 1 hour).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Record OD 600 and add equivalent of 1 &mu;L of OD 2.0 cells to 1mL PBS with 1 mg/mL BSA and specified flourophore concentration (0-100 nM) and allow time to bind (30 minutes to 1 hour).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Preincubate second set of samples in 1 &mu;M biotin to test for nonspecific binding of the fluorophore to the cells</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Preincubate second set of samples in 1 &mu;M biotin to test for nonspecific binding of the fluorophore to the cells</div></td></tr>
</table>Roberehttp://2009.igem.org/wiki/index.php?title=Team:Washington/Notebook/Flow_Cytometry&diff=125261&oldid=prevRobere: /* Flow Cytometry */2009-10-20T06:59:36Z<p><span class="autocomment">Flow Cytometry</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Flow Cytometry ====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Flow Cytometry ====</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Set up overnights of parts <del class="diffchange diffchange-inline">48</del>-<del class="diffchange diffchange-inline">51 </del>in terrific broth (TB). Let grow overnight at 37 &deg;C.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Set up overnights of parts <ins class="diffchange diffchange-inline">J36848</ins>-<ins class="diffchange diffchange-inline">J36851 </ins>in terrific broth (TB). Let grow overnight at 37 &deg;C.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Dilute 20 &mu;L overnight into 1 mL TB and grow at 37 &deg;C until OD 0.3 to OD 0.8.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Dilute 20 &mu;L overnight into 1 mL TB and grow at 37 &deg;C until OD 0.3 to OD 0.8.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add IPTG to 1 mM concentration and let grow for minimum of four hours at room temperature (~23 &deg;C).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add IPTG to 1 mM concentration and let grow for minimum of four hours at room temperature (~23 &deg;C).</div></td></tr>
</table>Roberehttp://2009.igem.org/wiki/index.php?title=Team:Washington/Notebook/Flow_Cytometry&diff=125255&oldid=prevRobere: /* Flow Cytometry */2009-10-20T06:59:01Z<p><span class="autocomment">Flow Cytometry</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Record cytometry data with 100 &mu;L of each sample.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Record cytometry data with 100 &mu;L of each sample.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Centrifuge samples, carefully pipette off supernatant to ~20 &mu;L to avoid disturbing loose pellets, and resuspend samples in 1mL PBS + BSA</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Centrifuge samples, carefully pipette off supernatant to ~20 &mu;L to avoid disturbing loose pellets, and resuspend samples in 1mL PBS + BSA</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#*: Spin beads at <del class="diffchange diffchange-inline">1000x </del>g for 1 minute</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#*: Spin beads at <ins class="diffchange diffchange-inline">1000 x </ins>g for 1 minute</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#*: Spin cells at 17,<del class="diffchange diffchange-inline">000x </del>g for 1 minute</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#*: Spin cells at 17,<ins class="diffchange diffchange-inline">000 x </ins>g for 1 minute</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Record cytometry data again with reduced fluorescence background</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Record cytometry data again with reduced fluorescence background</div></td></tr>
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</table>Roberehttp://2009.igem.org/wiki/index.php?title=Team:Washington/Notebook/Flow_Cytometry&diff=125248&oldid=prevRobere: /* Flow Cytometry */2009-10-20T06:58:03Z<p><span class="autocomment">Flow Cytometry</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Dilute 20 &mu;L overnight into 1 mL TB and grow at 37 &deg;C until OD 0.3 to OD 0.8.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Dilute 20 &mu;L overnight into 1 mL TB and grow at 37 &deg;C until OD 0.3 to OD 0.8.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add IPTG to 1 mM concentration and let grow for minimum of four hours at room temperature (~23 &deg;C).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add IPTG to 1 mM concentration and let grow for minimum of four hours at room temperature (~23 &deg;C).</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Record OD 600 and add equivalent of 1 &mu;L of OD 2.0 cells to 1mL PBS with 1 mg/mL BSA and specified flourophore concentration (0-100 nM) and allow time to bind (<del class="diffchange diffchange-inline">~</del>1 hour).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Record OD 600 and add equivalent of 1 &mu;L of OD 2.0 cells to 1mL PBS with 1 mg/mL BSA and specified flourophore concentration (0-100 nM) and allow time to bind (<ins class="diffchange diffchange-inline">30 minutes to </ins>1 hour).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Preincubate second set of samples in 1 &mu;M biotin to test for nonspecific binding of the fluorophore to the cells</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Preincubate second set of samples in 1 &mu;M biotin to test for nonspecific binding of the fluorophore to the cells</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Similarly incubate streptavidin-coated beads with fluorophore in PBS + BSA.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Similarly incubate streptavidin-coated beads with fluorophore in PBS + BSA.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Record cytometry data with 100 &mu;L of each sample.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Record cytometry data with 100 &mu;L of each sample.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Centrifuge samples, carefully pipette off supernatant, and resuspend samples in 1mL PBS + BSA</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Centrifuge samples, carefully pipette off supernatant <ins class="diffchange diffchange-inline">to ~20 &mu;L to avoid disturbing loose pellets</ins>, and resuspend samples in 1mL PBS + BSA</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#: Spin beads at 1000x g for 1 minute</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#<ins class="diffchange diffchange-inline">*</ins>: Spin beads at 1000x g for 1 minute</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#: Spin cells at 17,000x g for 1 minute</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#<ins class="diffchange diffchange-inline">*</ins>: Spin cells at 17,000x g for 1 minute</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Record cytometry data again with reduced fluorescence background</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Record cytometry data again with reduced fluorescence background</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Team:Washington/Templates/Footer}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Team:Washington/Templates/Footer}}</div></td></tr>
</table>Roberehttp://2009.igem.org/wiki/index.php?title=Team:Washington/Notebook/Flow_Cytometry&diff=125219&oldid=prevRobere: /* Flow Cytometry */2009-10-20T06:55:39Z<p><span class="autocomment">Flow Cytometry</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Flow Cytometry ====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Flow Cytometry ====</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Set up overnights of parts 48-51. Let grow overnight.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Set up overnights of parts 48-51 <ins class="diffchange diffchange-inline">in terrific broth (TB)</ins>. Let grow overnight <ins class="diffchange diffchange-inline">at 37 &deg;C</ins>.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Dilute <del class="diffchange diffchange-inline">1 ul </del>overnight into <del class="diffchange diffchange-inline">1ml</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Dilute <ins class="diffchange diffchange-inline">20 &mu;L </ins>overnight into <ins class="diffchange diffchange-inline">1 mL TB and grow at 37 &deg;C until OD 0.3 to OD 0.8.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Add <del class="diffchange diffchange-inline">1 mm </del>IPTG and let grow for four hours</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Add IPTG <ins class="diffchange diffchange-inline">to 1 mM concentration </ins>and let grow for <ins class="diffchange diffchange-inline">minimum of </ins>four hours <ins class="diffchange diffchange-inline">at room temperature (~23 &deg;C).</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># <del class="diffchange diffchange-inline">After </del>cells <del class="diffchange diffchange-inline">have grown up, place in </del>flourophore (<del class="diffchange diffchange-inline">1 um</del>) and allow time to bind (1 hour)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># <ins class="diffchange diffchange-inline">Record OD 600 and add equivalent of 1 &mu;L of OD 2.0 </ins>cells <ins class="diffchange diffchange-inline">to 1mL PBS with 1 mg/mL BSA and specified </ins>flourophore <ins class="diffchange diffchange-inline">concentration </ins>(<ins class="diffchange diffchange-inline">0-100 nM</ins>) and allow time to bind (<ins class="diffchange diffchange-inline">~</ins>1 hour)<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># <del class="diffchange diffchange-inline">Also place </del>1 <del class="diffchange diffchange-inline">ul </del>beads in <del class="diffchange diffchange-inline">1 ml along </del>with <del class="diffchange diffchange-inline">1 ul flourophore</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># <ins class="diffchange diffchange-inline">Preincubate second set of samples in </ins>1 <ins class="diffchange diffchange-inline">&mu;M biotin to test for nonspecific binding of the fluorophore to the cells</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># <del class="diffchange diffchange-inline">Allow beads to bind to flourophore</del>, <del class="diffchange diffchange-inline">then spin beads down</del>, <del class="diffchange diffchange-inline">remove supernatent </del>and <del class="diffchange diffchange-inline">replace with </del>1 <del class="diffchange diffchange-inline">ml water.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"># Similarly incubate streptavidin-coated </ins>beads <ins class="diffchange diffchange-inline">with fluorophore </ins>in <ins class="diffchange diffchange-inline">PBS + BSA.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># <del class="diffchange diffchange-inline">Read the samples through the flow cytometer</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"># Record cytometry data </ins>with <ins class="diffchange diffchange-inline">100 &mu;L of each sample</ins>.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># <ins class="diffchange diffchange-inline">Centrifuge samples</ins>, <ins class="diffchange diffchange-inline">carefully pipette off supernatant</ins>, and <ins class="diffchange diffchange-inline">resuspend samples in 1mL PBS + BSA</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#: Spin beads at 1000x g for </ins>1 <ins class="diffchange diffchange-inline">minute</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#<ins class="diffchange diffchange-inline">: Spin cells at 17,000x g for 1 minute</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"># Record cytometry data again with reduced fluorescence background</ins></div></td></tr>
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</table>Roberehttp://2009.igem.org/wiki/index.php?title=Team:Washington/Notebook/Flow_Cytometry&diff=100027&oldid=prevDunbar at 05:11, 15 October 20092009-10-15T05:11:40Z<p></p>
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</table>Dunbarhttp://2009.igem.org/wiki/index.php?title=Team:Washington/Notebook/Flow_Cytometry&diff=100010&oldid=prevDunbar at 05:07, 15 October 20092009-10-15T05:07:57Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Flow Cytometry ====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Flow Cytometry ====</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Set up overnights of parts 48-51. Let grow overnight.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Set up overnights of parts 48-51. Let grow overnight.</div></td></tr>
</table>Dunbarhttp://2009.igem.org/wiki/index.php?title=Team:Washington/Notebook/Flow_Cytometry&diff=99917&oldid=prevDunbar: New page: ==== Flow Cytometry ==== # Set up overnights of parts 48-51. Let grow overnight. # Dilute 1 ul overnight into 1ml # Add 1 mm IPTG and let grow for four hours # After cells have grown up, p...2009-10-15T04:03:10Z<p>New page: ==== Flow Cytometry ==== # Set up overnights of parts 48-51. Let grow overnight. # Dilute 1 ul overnight into 1ml # Add 1 mm IPTG and let grow for four hours # After cells have grown up, p...</p>
<p><b>New page</b></p><div>==== Flow Cytometry ====<br />
# Set up overnights of parts 48-51. Let grow overnight.<br />
# Dilute 1 ul overnight into 1ml<br />
# Add 1 mm IPTG and let grow for four hours<br />
# After cells have grown up, place in flourophore (1 um) and allow time to bind (1 hour)<br />
# Also place 1 ul beads in 1 ml along with 1 ul flourophore.<br />
# Allow beads to bind to flourophore, then spin beads down, remove supernatent and replace with 1 ml water.<br />
# Read the samples through the flow cytometer</div>Dunbar