Team:Washington/Project/Opda

From 2009.igem.org

(Difference between revisions)

Revision as of 06:58, 13 October 2009

Target Vector	Secretion System	Display System

Opda is an organophosphate-degrading enzyme from Agrobacterium radiobacter. It is capable of degrading a wide range of organophosphates, most notably pesticides that are poisonous to humans, such as paraoxon. We chose to biobrick this enzyme for a number of reasons. First and foremost, this enzyme is easy to assay for since it can hydrolyze substrates (e.g. paraoxon) and form a bright yellow product. This yellow product would make it easy to see that the Opda was present and functioning in our system. And secondly, Opda is a very useful enzyme that could have applications in future iGEM and other synthetic biology projects, so its presence in the Standard Registry of Biological Parts is beneficial.

Revision as of 06:38, 13 October 2009 (view source) Jeff (Talk \| contribs) ← Older edit		Revision as of 06:58, 13 October 2009 (view source) Jeff (Talk \| contribs) Newer edit →
Line 10:		Line 10:


-	~~opda!!~~	+	Opda is an organophosphate-degrading enzyme from Agrobacterium radiobacter. It is capable of degrading a wide range of organophosphates, most notably pesticides that are poisonous to humans, such as paraoxon. We chose to biobrick this enzyme for a number of reasons. First and foremost, this enzyme is easy to assay for since it can hydrolyze substrates (e.g. paraoxon) and form a bright yellow product. This yellow product would make it easy to see that the Opda was present and functioning in our system. And secondly, Opda is a very useful enzyme that could have applications in future iGEM and other synthetic biology projects, so its presence in the Standard Registry of Biological Parts is beneficial.