Team:Washington/Project/Opda

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=Opda=
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==Background==
Opda is an organophosphate-degrading enzyme from Agrobacterium radiobacter. It is capable of degrading a wide range of organophosphates, most notably pesticides that are poisonous to humans, such as paraoxon. We chose to biobrick this enzyme for a number of reasons. First and foremost, this enzyme is easy to assay for since it can hydrolyze substrates (e.g. paraoxon) and form a bright yellow product. This yellow product would make it easy to see that the Opda was present and functioning in our system.  And secondly, Opda is a very useful enzyme that could have applications in future iGEM and other synthetic biology projects, so its presence in the Standard Registry of Biological Parts is beneficial.
Opda is an organophosphate-degrading enzyme from Agrobacterium radiobacter. It is capable of degrading a wide range of organophosphates, most notably pesticides that are poisonous to humans, such as paraoxon. We chose to biobrick this enzyme for a number of reasons. First and foremost, this enzyme is easy to assay for since it can hydrolyze substrates (e.g. paraoxon) and form a bright yellow product. This yellow product would make it easy to see that the Opda was present and functioning in our system.  And secondly, Opda is a very useful enzyme that could have applications in future iGEM and other synthetic biology projects, so its presence in the Standard Registry of Biological Parts is beneficial.
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==Experiments==

Revision as of 06:59, 13 October 2009

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Target Vector Secretion System Display System

Opda

Background

Opda is an organophosphate-degrading enzyme from Agrobacterium radiobacter. It is capable of degrading a wide range of organophosphates, most notably pesticides that are poisonous to humans, such as paraoxon. We chose to biobrick this enzyme for a number of reasons. First and foremost, this enzyme is easy to assay for since it can hydrolyze substrates (e.g. paraoxon) and form a bright yellow product. This yellow product would make it easy to see that the Opda was present and functioning in our system. And secondly, Opda is a very useful enzyme that could have applications in future iGEM and other synthetic biology projects, so its presence in the Standard Registry of Biological Parts is beneficial.

==Experiments==