Target Vector

Background

The Target Vector is designed as the construct that will create a fusion protein, tagging afp for use in the IPP system. The first key feature of the Target's design is the NheI restriction site. NheI is compatible with XbaI and SpeI, meaning that a biobrick digested at the X and S sites can be ligated into the Target Vector at the NheI site (for detailed protocol see here!!!). At the N-terminus of the Target is the display (aka Nano) tag, which is a 15 amino acid sequence that binds afp to streptavidin or some analogous protein. At the C-terminus of the Target is the 180 amino acid Secretion tag (aka prtB) that is recognized and secreted to the extracellular space by the IPP's secretion system.

Experiments