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Team:Waterloo/Notebook/Protocols/Inoculation
From 2009.igem.org
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(New page: {{Team:Waterloo/NavBar}} <big><b><u>Inoculation Protocol </u></b></big><br> <b>Materials </b><br> DNA Samples <br> Ampicillin (2µL/sample)<br> Kanamycin (2.5µL/sample)<br> Liquid LB Me...) |
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- Pipette the correct amount of antibiotic into the corresponding DNA tubes. 2 µL for ApR, 2.5 µL KmR. <br> | - Pipette the correct amount of antibiotic into the corresponding DNA tubes. 2 µL for ApR, 2.5 µL KmR. <br> | ||
- Dip the sterile stick into the DNA and into the corresponding media tube.<br> | - Dip the sterile stick into the DNA and into the corresponding media tube.<br> | ||
| - | 3. Incubate at 37 degrees | + | 3. Incubate at 37 degrees Celsius overnight on the agitator.<br> |
Latest revision as of 02:59, 22 October 2009
Inoculation Protocol
Materials
DNA Samples
Ampicillin (2µL/sample)
Kanamycin (2.5µL/sample)
Liquid LB Media (1 tube/sample)
Instructions
1. Label all tubes.
2. Do the following in the flow hood:
- Pipette the correct amount of antibiotic into the corresponding DNA tubes. 2 µL for ApR, 2.5 µL KmR.
- Dip the sterile stick into the DNA and into the corresponding media tube.
3. Incubate at 37 degrees Celsius overnight on the agitator.
