Team:Waterloo/Project

From 2009.igem.org

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(Part 2)
(The Experiments)
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== The Experiments ==
== The Experiments ==
===Cassette Exchange===
===Cassette Exchange===
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====BBa Donor Plasmid====
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=====BBa Donor Plasmid=====
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====Landing Pad Strain====
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=====Landing Pad Strain=====
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====Integrase expression plasmid====
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=====Integrase expression plasmid=====
===Non-crossreactive <i>att</i> sites===
===Non-crossreactive <i>att</i> sites===
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====Integrase Mechanism====
+
=====Integrase Mechanism=====
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====Design of new <i>att</i> sites====
+
=====Design of new <i>att</i> sites=====
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====Design of experiment to characterize new <i>att</i> sites====
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=====Design of experiment to characterize new <i>att</i> sites=====
=== Part 3 ===
=== Part 3 ===
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== Results ==
== Results ==

Revision as of 05:02, 11 October 2009


Home The Team The Project Parts Submitted to the Registry Modeling Notebook

(Or you can choose different headings. But you must have a team page, a project page, and a notebook page.)


Contents

Chromobricks: A Platform for Chromosome Engineering with BioBricks

The aim of our project is to develop a fully-featured platform for chromosome engineering, allowing the in vivo assembly of a synthetic chromosome from interchangeable parts, followed by selective degradation of the native chromosome. We have designed a proof-of-concept for chromosome-building that will use the site-specific integrase of phage ΦC31 to integrate a BioBrick into a defined locus of the E. coli genome. Six pairs of integrase-targeted att sites have been designed to be non-cross-reactive in order to support repeatable cassette-exchange reactions for chromosome building. We have also written software to model the integrase-mediated rearrangement of DNA molecules containing att sites, to aid the design of more elaborate chromosome-building systems. To selectively degrade the native chromosome we designed a nuclease-based, inducible genome-degradation system. In its simplest form, our system can be used to integrate biological devices into a chromosome in situations requiring stable copy number and selection-free maintenance.

Project Details

The Experiments

Cassette Exchange

BBa Donor Plasmid
Landing Pad Strain
Integrase expression plasmid

Non-crossreactive att sites

Integrase Mechanism
Design of new att sites
Design of experiment to characterize new att sites

Part 3

Results