Team:Waterloo/Project

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Home The Team The Project Parts Submitted to the Registry Modeling Notebook

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Contents

Chromobricks: A Platform for Chromosome Engineering with BioBricks

The aim of our project is to develop a fully-featured platform for chromosome engineering, allowing the in vivo 

assembly of a synthetic chromosome from interchangeable parts, followed by selective degradation of the native
chromosome. We have designed a proof-of-concept for chromosome-building that will use the site-specific integrase of
phage ΦC31 to integrate a BioBrick into a defined locus of the E. coli genome. Six pairs of integrase-targeted

att sites have been designed to be non-cross-reactive in order to support repeatable cassette-exchange 

reactions for chromosome building. We have also written software to model the integrase-mediated rearrangement of DNA
molecules containing att sites, to aid the design of more elaborate chromosome-building systems. To selectively

degrade the native chromosome we designed a nuclease-based, inducible genome-degradation system. In its simplest form,
our system can be used to integrate biological devices into a chromosome in situations requiring stable copy number
and selection-free maintenance.

Project Details

Part 2

The Experiments

Part 3

Results