Team:Wisconsin-Madison/Notebook Protocols

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Protocols

Plasmid Preparation by Alkaline Lysis Plasmid Preparation by Boiling Lysis Transformation of Plasmids into Competant Cells Preparation of Competent Cells Cloning Protocols Polymerase Chain Reaction (PCR) Ligation Digestion Inoculation of Plasmids in Liquid Media Inoculation of Cyanobacteria Loading Gel Protocol Plasmid Clean-up Gel Extraction from Agar Sequencing Reaction Colony Polymerase Chain Reaction (PCR)