Team:uOttawa/Parts

From 2009.igem.org

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         <a href="https://2009.igem.org/Team:uOttawa/Project">Project</a></li>
         <a href="https://2009.igem.org/Team:uOttawa/Project">Project</a></li>
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         <a href="https://2009.igem.org/Team:uOttawa/Parts">Parts</a></li>
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         <a href="https://2009.igem.org/Team:uOttawa/Biobricks">Biobricks</a></li>
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         <a href="https://2009.igem.org/Team:uOttawa/Modeling">Modeling</a></li>
         <a href="https://2009.igem.org/Team:uOttawa/Modeling">Modeling</a></li>
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<a href="https://2009.igem.org/Team:uOttawa/Notebook">Notebook</a></li>
<a href="https://2009.igem.org/Team:uOttawa/Notebook">Notebook</a></li>
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       <li>
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        <li> <a href="https://2009.igem.org/Team:uOttawa/Acknowlegments">Acknowledgments</a></li>
         <a href="https://2009.igem.org/Team:uOttawa/Project"">FR</a></li>
         <a href="https://2009.igem.org/Team:uOttawa/Project"">FR</a></li>
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     </div>
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     <h1 id="header">
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       <a href="/">uOttawa IGEM2009</a></h1>
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       uOttawa IGEM2009</h1>
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         <h2>Who we are</h2>
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         <h2>Biobrick </h2>
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        We are a team consisting of 1 scientific advisors and 14 undergraduate students from the University of Ottawa. This is the second year that the University of Ottawa has recruited a team to participate in the iGem competition. Our team members have a variety of backgrounds and areas of expertise. Feel free to take a look at our team page to find out more about us.
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We have contributed to the iGEM biobrick registry by submitting several parts which can be used in eukaryotic organisms, namely in Yeast and Mammalian cells. The Gal 1/10 divergent promoter is essentially composed of two promoters and is capable of driving gene expression bidirectionally. Gene expression is driven in one direction by the Gal 1 promoter and by the Gal 10 promoter in the other direction, both promoters are inducible by galactose present in the cell medium. The construct was isolated from the pRS4T123 plasmid, generously donated to us by the James Collins Lab. Due  to it's divergent design, the dual promoter is compact and thus easy to work with. Hilary Phenix and Vida Adebi helped us with flow cytometry and they also generated a yeast strain containing a Gal 1/10 divergent promoter, with Gal 10 driving GFP expression. We characterized the Gal 10 component of the promoter in yeast by performing a dose response experiment where we induced the yeast with various concentrations of galactose and measured the resulting GFP output using a flow cytometer. We also contributed a CYC1 terminator containing a stop codon to the registry, a commonly used terminator sequence in yeast which was also isolated form the pRS4T123 plasmid . Including a stop codon with the terminator allows this construct to be fused with proteins in the biofusion format (assembly standard 23). We also submitted a CMV promoter, naturally found in the Cytomegalovirus. The submitted promoter was isolated from a plasmid that was created in our lab, pCMV is a constitutive promoter commonly used in mammalian cloning vectors. We submitted these parts in a effort provide at least some basic components to the registry that can be used by future iGEM teams who wish to engineer eukaryotic cells
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<h2> Advisors</h2>
 
<p> </p>
<p> </p>
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<h2> the team</h2>
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<p> </P<p>
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<h2>Tech museum on the 5th of May</h2>
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          <table width="100%" border="0" cellspacing="2" cellpadding="2">
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            <td><a  href="http://www.ipm-int.org/boxmode/image/museum/DSCF1238.JPG" rel="lightbox[museum]" title="Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages, and more recently with desktop publishing software like Aldus PageMaker including versions of Lorem Ipsum."><img  src="http://www.ipm-int.org/boxmode/image/museum/thumbs-1.jpg" width="85" height="85" alt=""  /></a></td>
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            <td><a href="http://www.ipm-int.org/boxmode/image/museum/DSCF1241.JPG" rel="lightbox[museum]"><img src="http://www.ipm-int.org/boxmode/image/museum/thumbs-2.jpg" width="85" height="85" alt="" /></a></td>
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            <td><a href="http://www.ipm-int.org/boxmode/image/museum/DSCF1242.JPG" rel="lightbox[museum]"><img src="http://www.ipm-int.org/boxmode/image/museum/thumbs-3.jpg" width="85" height="85" alt="" /></a></td>
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            <td><a href="http://www.ipm-int.org/boxmode/image/museum/DSCF1243.JPG" rel="lightbox[museum]"><img src="http://www.ipm-int.org/boxmode/image/museum/thumbs-4.jpg" width="85" height="85" alt="" /></a></td>
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            <td><a href="http://www.ipm-int.org/boxmode/image/museum/DSCF1244.JPG" rel="lightbox[museum]"><img src="http://www.ipm-int.org/boxmode/image/museum/thumbs-5.jpg" width="85" height="85" alt="" /></a></td>
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            <td><a href="http://www.ipm-int.org/boxmode/image/museum/DSCF1247.JPG" rel="lightbox[museum]"><img src="http://www.ipm-int.org/boxmode/image/museum/thumbs-6.jpg" width="85" height="85" alt="" /></a></td>
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            <td>&nbsp;</td>
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            <td>&nbsp;</td>
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          </tr>
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        </table>
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        </p>
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        <p class="info">
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          |<a href="#">March 26th</a>
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       </div>
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       <div id="sidebar">
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         <h3>Previous Posts</h3>
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         <h3>uOttawa iGEM2009</h3>
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           <a href="#">Lorem ipsum</a></li>
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           <a href="http://www.ipm-int.org/boxmode/pdf/Ethics.pdf" target="_parent">ETHICS</a></li>
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           <a href="#">Lorem ipsum</a></li>
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           <a href="http://www.ipm-int.org/boxmode/pdf/Security.pdf">SECURITY</a></li>
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         <h3>Links</h3>
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<h3>Health</h3>
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         <ul>
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          <li>  <a href="http://www.ipm-int.org/boxmode/pdf/Probiotics in the Food Industry.pdf" target="_parent">probiotics</a></li>
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          <li>  <a href="http://www.ipm-int.org/boxmode/pdf/Acetobacter_xylinum.pdf">Acetobacter xylinum</a></li>
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          <li>  <a href="http://www.ipm-int.org/boxmode/pdf/Obesity.pdf">Obesity</a></li>
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        </ul>
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      <h3>Sponsors</h3>
         <ul>
         <ul>
           <li>
           <li>
           <a href="http://www.uottawa.ca">uottawa</a></li>
           <a href="http://www.uottawa.ca">uottawa</a></li>
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           <a href="#">Link Item 2</a></li>
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           <a href="http://www.medicine.uottawa.ca/">Faculty of medicine</a></li>
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           <a href="#">Link Item 3</a></li>
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           <a href="http://www.engineering.uottawa.ca/">Faculty of engineering</a></li>
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           <a href="#">Link Item 4</a></li>
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           <a href="http://www.uottawa.ca/research/">VP research</a></li>
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          <a href="http://www.epocal.com/">Epocal</a></li>
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          <li>
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          <a href="http://www.medicine.uottawa.ca/crem/eng/">CREM-CRME</a></li>
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          <li>
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          <a href="http://www.gehealthcare.com/caen/">GE healthcare</a></li>
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         </ul>
         </ul>
         <h3>Latest Events</h3>
         <h3>Latest Events</h3>
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     <div id="footer">
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         &copy; 2006 Site Title, all rights reserved.<br />
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         &copy; 2009 uOttawa iGEM, all rights reserved.<br />
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         <a href="http://www.example.com/" title="Powered by .."> uottawa igem2009</a>,  
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Latest revision as of 19:03, 21 October 2009

uOttawa IGEM2009

uOttawa IGEM2009

Biobrick

We have contributed to the iGEM biobrick registry by submitting several parts which can be used in eukaryotic organisms, namely in Yeast and Mammalian cells. The Gal 1/10 divergent promoter is essentially composed of two promoters and is capable of driving gene expression bidirectionally. Gene expression is driven in one direction by the Gal 1 promoter and by the Gal 10 promoter in the other direction, both promoters are inducible by galactose present in the cell medium. The construct was isolated from the pRS4T123 plasmid, generously donated to us by the James Collins Lab. Due to it's divergent design, the dual promoter is compact and thus easy to work with. Hilary Phenix and Vida Adebi helped us with flow cytometry and they also generated a yeast strain containing a Gal 1/10 divergent promoter, with Gal 10 driving GFP expression. We characterized the Gal 10 component of the promoter in yeast by performing a dose response experiment where we induced the yeast with various concentrations of galactose and measured the resulting GFP output using a flow cytometer. We also contributed a CYC1 terminator containing a stop codon to the registry, a commonly used terminator sequence in yeast which was also isolated form the pRS4T123 plasmid . Including a stop codon with the terminator allows this construct to be fused with proteins in the biofusion format (assembly standard 23). We also submitted a CMV promoter, naturally found in the Cytomegalovirus. The submitted promoter was isolated from a plasmid that was created in our lab, pCMV is a constitutive promoter commonly used in mammalian cloning vectors. We submitted these parts in a effort provide at least some basic components to the registry that can be used by future iGEM teams who wish to engineer eukaryotic cells