http://2009.igem.org/wiki/index.php?title=Team:uOttawa/Project&feed=atom&action=history
Team:uOttawa/Project - Revision history
2024-03-28T18:01:39Z
Revision history for this page on the wiki
MediaWiki 1.16.5
http://2009.igem.org/wiki/index.php?title=Team:uOttawa/Project&diff=166756&oldid=prev
Bbuildman at 02:54, 22 October 2009
2009-10-22T02:54:38Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:54, 22 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 88:</td>
<td colspan="2" class="diff-lineno">Line 88:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The constitutive P45 promoter, Nisin resistance and RepA were all PCR amplified out of the food grade cloning vector PLEB590 with added restriction sites, digested, and ligated to the RFP/terminator biobrick to create the plasmid expression RFP construct.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The constitutive P45 promoter, Nisin resistance and RepA were all PCR amplified out of the food grade cloning vector PLEB590 with added restriction sites, digested, and ligated to the RFP/terminator biobrick to create the plasmid expression RFP construct.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">h2>Insert the RFP</del>/<del class="diffchange diffchange-inline">Terminator</del>/<del class="diffchange diffchange-inline">P45</del>/<del class="diffchange diffchange-inline">Nis</del>/<del class="diffchange diffchange-inline">RepA vector map here</del>.<del class="diffchange diffchange-inline"><</del>/<del class="diffchange diffchange-inline">h2</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">img src="http:</ins>//<ins class="diffchange diffchange-inline">www.ipm-int.org</ins>/<ins class="diffchange diffchange-inline">boxmode</ins>/<ins class="diffchange diffchange-inline">image/figure/RFP-Plasmid</ins>.<ins class="diffchange diffchange-inline">jpg" width="443" height="393" </ins>/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We plan to transform the constructs into E. coli. in parallel with transformation into L.plantarum. We will do this to ensure expression of our construct, since L. plantarum and A. xylinum have different gram stain. This variation in membrane structure may affect cellulose synthase expression, since the sugar polymer must be extruded through membrane pores. E. coli and A. xylinum are both gram negative, so we increase the chance of cellulose synthase expression by transforming into E. coli in addition to Lactobacillus.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We plan to transform the constructs into E. coli. in parallel with transformation into L.plantarum. We will do this to ensure expression of our construct, since L. plantarum and A. xylinum have different gram stain. This variation in membrane structure may affect cellulose synthase expression, since the sugar polymer must be extruded through membrane pores. E. coli and A. xylinum are both gram negative, so we increase the chance of cellulose synthase expression by transforming into E. coli in addition to Lactobacillus.</p></div></td></tr>
</table>
Bbuildman
http://2009.igem.org/wiki/index.php?title=Team:uOttawa/Project&diff=166553&oldid=prev
Bbuildman at 02:50, 22 October 2009
2009-10-22T02:50:28Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:50, 22 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 78:</td>
<td colspan="2" class="diff-lineno">Line 78:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Takala, T., Saris, P., (2002): A food-grade cloning vector for lactic acid bacteria based on the nisin immunity gene nisI. Applied Microbiology and Biotechnology, 59:467-468.</p></em></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Takala, T., Saris, P., (2002): A food-grade cloning vector for lactic acid bacteria based on the nisin immunity gene nisI. Applied Microbiology and Biotechnology, 59:467-468.</p></em></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">h2>Insert cellulose synthase plasmid expression vector map here</del>.<del class="diffchange diffchange-inline"><</del>/<del class="diffchange diffchange-inline">h2</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">img src="http://www.ipm-int.org/boxmode/image/figure/Synthase-Plasmid-Expression</ins>.<ins class="diffchange diffchange-inline">jpg" width="399" height="363" </ins>/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our finished genomic-expression construct replaces the gram-positive origin of replication with the mv4 bacteriophage integrase enzyme to integrate the cellulose synthase operon directly into the L. plantarum genome for increased stability and expression levels.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our finished genomic-expression construct replaces the gram-positive origin of replication with the mv4 bacteriophage integrase enzyme to integrate the cellulose synthase operon directly into the L. plantarum genome for increased stability and expression levels.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The integrase coding int gene and bacteriophage attP site were PCR amplified from the bacteriophage integrative vector PMC1 with added restriction sites, digested and ligated to the P45 promoter/Nisin resistance. This will soon be ligated to the cellulose synthase to create the genomic expression synthase construct. These constructs will then be transformed through an electroporation procedure into Lactobacillus plantarum.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The integrase coding int gene and bacteriophage attP site were PCR amplified from the bacteriophage integrative vector PMC1 with added restriction sites, digested and ligated to the P45 promoter/Nisin resistance. This will soon be ligated to the cellulose synthase to create the genomic expression synthase construct. These constructs will then be transformed through an electroporation procedure into Lactobacillus plantarum.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">h2>Insert cellulose synthase genomic expression vector map here</del>.<del class="diffchange diffchange-inline"><</del>/<del class="diffchange diffchange-inline">h2</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">img src="http://www.ipm-int.org/boxmode/image/figure/Synthase-Genomic-Expression</ins>.<ins class="diffchange diffchange-inline">jpg" alt="Genomic" width="399" height="333" </ins>/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The last construct will characterize the expression of the P45 promoter by fusing it to the reporter RFP. This construct contains the P45 promoter, nisin antibiotic resistance and RepA, the origin of replication, all of which are ligated to the RFP/terminator biobrick found in the iGEM registry of parts.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The last construct will characterize the expression of the P45 promoter by fusing it to the reporter RFP. This construct contains the P45 promoter, nisin antibiotic resistance and RepA, the origin of replication, all of which are ligated to the RFP/terminator biobrick found in the iGEM registry of parts.</p></div></td></tr>
</table>
Bbuildman
http://2009.igem.org/wiki/index.php?title=Team:uOttawa/Project&diff=166214&oldid=prev
Jjulus at 02:43, 22 October 2009
2009-10-22T02:43:51Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:43, 22 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 138:</td>
<td colspan="2" class="diff-lineno">Line 138:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="http://www.ipm-int.org/boxmode/pdf/Glucose.pdf">Glucose and cellulose digestion</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="http://www.ipm-int.org/boxmode/pdf/Glucose.pdf">Glucose and cellulose digestion</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline"><li></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> <a href="http://www.ipm-int.org/boxmode/pdf/Probiotics in the Food Industry.pdf" target="_parent">Probiotics</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> <a href="http://www.ipm-int.org/boxmode/pdf/Probiotics in the Food Industry.pdf" target="_parent">Probiotics</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> <a href="http://www.ipm-int.org/boxmode/pdf/Obesity.pdf">Obesity</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> <a href="http://www.ipm-int.org/boxmode/pdf/Obesity.pdf">Obesity</a></li></div></td></tr>
</table>
Jjulus
http://2009.igem.org/wiki/index.php?title=Team:uOttawa/Project&diff=165394&oldid=prev
Jjulus at 02:26, 22 October 2009
2009-10-22T02:26:07Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:26, 22 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 128:</td>
<td colspan="2" class="diff-lineno">Line 128:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="http://www.ipm-int.org/boxmode/pdf/Security.pdf">SECURITY</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="http://www.ipm-int.org/boxmode/pdf/Security.pdf">SECURITY</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <a href="http://www.ipm-int.org/boxmode/pdf/Safety.pdf">Project Safety</a></li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><li> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="http://www.ipm-int.org/boxmode/pdf/business.pdf">uOttawa iGEM integrating business and</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="http://www.ipm-int.org/boxmode/pdf/business.pdf">uOttawa iGEM integrating business and</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>science</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>science</a></li></div></td></tr>
</table>
Jjulus
http://2009.igem.org/wiki/index.php?title=Team:uOttawa/Project&diff=160980&oldid=prev
Bbuildman at 00:08, 22 October 2009
2009-10-22T00:08:51Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 00:08, 22 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 28:</td>
<td colspan="2" class="diff-lineno">Line 28:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h1 id="header">uOttawa <del class="diffchange diffchange-inline">IGEM2009</del></h1></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h1 id="header">uOttawa <ins class="diffchange diffchange-inline">iGEM2009</ins></h1></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="wrap"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="wrap"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="page"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="page"></div></td></tr>
</table>
Bbuildman
http://2009.igem.org/wiki/index.php?title=Team:uOttawa/Project&diff=158899&oldid=prev
Bbuildman at 23:05, 21 October 2009
2009-10-21T23:05:44Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 23:05, 21 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 122:</td>
<td colspan="2" class="diff-lineno">Line 122:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <hr class="hide" /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <hr class="hide" /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="sidebar"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="sidebar"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><h3>uOttawa iGEM2009</h3></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><h3>uOttawa iGEM2009</h3></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 128:</td>
<td colspan="2" class="diff-lineno">Line 128:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="http://www.ipm-int.org/boxmode/pdf/Security.pdf">SECURITY</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="http://www.ipm-int.org/boxmode/pdf/Security.pdf">SECURITY</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline"><li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <a href="http://www.ipm-int.org/boxmode/pdf/business.pdf">uOttawa iGEM integrating business and</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">science</a></li></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Health</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Health</h3></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li> <a href="http://www.ipm-int.org/boxmode/pdf/Probiotics in the Food Industry.pdf" target="_parent"><del class="diffchange diffchange-inline">probiotics</del></a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <a href="http://www.ipm-int.org/boxmode/pdf/Glucose.pdf">Glucose and cellulose digestion</a></li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li> <a href="http://www.ipm-int.org/boxmode/pdf/Probiotics in the Food Industry.pdf" target="_parent"><ins class="diffchange diffchange-inline">Probiotics</a></li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li> <a href="http://www.ipm-int.org/boxmode/pdf/Obesity.pdf">Obesity</ins></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> <a href="http://www.ipm-int.org/boxmode/pdf/Acetobacter_xylinum.pdf">Acetobacter xylinum</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> <a href="http://www.ipm-int.org/boxmode/pdf/Acetobacter_xylinum.pdf">Acetobacter xylinum</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li> <del class="diffchange diffchange-inline"> </del><a href="http://www.ipm-int.org/boxmode/pdf/<del class="diffchange diffchange-inline">Obesity</del>.pdf"><del class="diffchange diffchange-inline">Obesity</del></a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li> <a href="http://www.ipm-int.org/boxmode/pdf/<ins class="diffchange diffchange-inline">lactobacillus_plantarum</ins>.pdf"><ins class="diffchange diffchange-inline">Lactobacillus</ins></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Sponsors</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Sponsors</h3></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td></tr>
</table>
Bbuildman
http://2009.igem.org/wiki/index.php?title=Team:uOttawa/Project&diff=154502&oldid=prev
Bbuildman at 20:41, 21 October 2009
2009-10-21T20:41:50Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:41, 21 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 108:</td>
<td colspan="2" class="diff-lineno">Line 108:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>Results</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>Results</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The team was successful in developing several protocols related to growing and working with both A. xylinum and L. plantarum. Both strains were novel to the Kaern Lab, so development of these protocols required extensive background research and trial and error. The protocols include <del class="diffchange diffchange-inline">a </del>xylinum media recipe, optimized miniprep <del class="diffchange diffchange-inline">protocols </del>for <del class="diffchange diffchange-inline">both strains </del>and <del class="diffchange diffchange-inline">growth </del>protocols for <del class="diffchange diffchange-inline">both strains</del>. <del class="diffchange diffchange-inline">These can all be found at </del>openwetware.org for future iGEM teams or other interested individuals. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In addition, iGEM uOttawa successfully managed to amplify the cellulose synthase codon (~10 kb). This required optimization of various parameters of the FinnZymes DyNAzyme Long-Range PCR kit. <p/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The team was successful in developing several protocols related to growing and working with both A. xylinum and L. plantarum. Both strains were novel to the Kaern Lab, so development of these protocols required extensive background research and trial and error. The protocols include <ins class="diffchange diffchange-inline">an A.</ins>xylinum media recipe <ins class="diffchange diffchange-inline">and growth protocol</ins>, <ins class="diffchange diffchange-inline">an </ins>optimized miniprep <ins class="diffchange diffchange-inline">protocol </ins>for <ins class="diffchange diffchange-inline">A. xylinum </ins>and <ins class="diffchange diffchange-inline">some </ins>protocols for <ins class="diffchange diffchange-inline">transformation into Lactobacillus plantarum</ins>. <ins class="diffchange diffchange-inline">All of these protocols have been posted on </ins>openwetware.org for future iGEM teams or other interested individuals. <ins class="diffchange diffchange-inline">The links to these protocols can all be found on the methods section of our Wiki, which provides links to openwetware.org </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In addition, iGEM uOttawa successfully managed to amplify the cellulose synthase codon (~10 kb) <ins class="diffchange diffchange-inline">seen confirmed on a gel below with a lambda hind III digest marker</ins>. This required optimization of various parameters of the FinnZymes DyNAzyme Long-Range PCR kit. <ins class="diffchange diffchange-inline"> </ins><p/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="center"><a href="http://www.ipm-int.org/boxmode/image/figure/Synthase Gel.bmp" rel="lightbox[figure]"><img src="http://www.ipm-int.org/boxmode/image/figure/thumbs-gel.png" width="300" height="300" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="center"><a href="http://www.ipm-int.org/boxmode/image/figure/Synthase Gel.bmp" rel="lightbox[figure]"><img src="http://www.ipm-int.org/boxmode/image/figure/thumbs-gel.png" width="300" height="300" alt="" /></a></p></div></td></tr>
</table>
Bbuildman
http://2009.igem.org/wiki/index.php?title=Team:uOttawa/Project&diff=153194&oldid=prev
Bbuildman at 19:59, 21 October 2009
2009-10-21T19:59:25Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:59, 21 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 17:</td>
<td colspan="2" class="diff-lineno">Line 17:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <body></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <body></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="navcontainer"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div id="navcontainer"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><ul id="navlist"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><ul id="navlist"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li> <a href="https://2009.igem.org/Team:uOttawa/Home" id="current">Home</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><a href="https://2009.igem.org/Team:uOttawa/Home" id="current">Home</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li> <a href="https://2009.igem.org/Team:uOttawa/Team">Team</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li> <a href="https://2009.igem.org/Team:uOttawa/Project">Project</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><a href="https://2009.igem.org/Team:uOttawa/Team">Team</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href="https://2009.igem.org/Team:uOttawa/Biobricks">Biobricks</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li> <a href="https://2009.igem.org/Team:uOttawa/Modeling">Modeling</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><a href="https://2009.igem.org/Team:uOttawa/Project">Project</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><li> <a href="https://2009.igem.org/Team:uOttawa/Notebook">Notebook</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li> <a href="https://2009.igem.org/Team:uOttawa/Acknowlegments">Acknowledgments</a></li></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><a href="https://2009.igem.org/Team:uOttawa/Biobricks">Biobricks</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><a href="https://2009.igem.org/Team:uOttawa/Modeling">Modeling</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><a href="https://2009.igem.org/Team:uOttawa/Notebook">Notebook</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
</table>
Bbuildman
http://2009.igem.org/wiki/index.php?title=Team:uOttawa/Project&diff=152945&oldid=prev
Bbuildman at 19:52, 21 October 2009
2009-10-21T19:52:07Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:52, 21 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 30:</td>
<td colspan="2" class="diff-lineno">Line 30:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://2009.igem.org/Team:uOttawa/Notebook">Notebook</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://2009.igem.org/Team:uOttawa/Notebook">Notebook</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <li></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <li> <a href="https://2009.igem.org/Team:uOttawa/Acknowlegments">Acknowledgments</a></li></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <a href="https://2009.igem.org/Team:uOttawa/Project"">FR</a></li></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><li></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <a href="https://2009.igem.org/Team:uOttawa/Project">EN</a></li></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
</table>
Bbuildman
http://2009.igem.org/wiki/index.php?title=Team:uOttawa/Project&diff=151773&oldid=prev
Jjulus at 19:08, 21 October 2009
2009-10-21T19:08:38Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:08, 21 October 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 25:</td>
<td colspan="2" class="diff-lineno">Line 25:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2009.igem.org/Team:uOttawa/Project">Project</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2009.igem.org/Team:uOttawa/Project">Project</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <a href="https://2009.igem.org/Team:uOttawa/<del class="diffchange diffchange-inline">Parts</del>"><del class="diffchange diffchange-inline">Parts</del></a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <a href="https://2009.igem.org/Team:uOttawa/<ins class="diffchange diffchange-inline">Biobricks</ins>"><ins class="diffchange diffchange-inline">Biobricks</ins></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2009.igem.org/Team:uOttawa/Modeling">Modeling</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="https://2009.igem.org/Team:uOttawa/Modeling">Modeling</a></li></div></td></tr>
</table>
Jjulus