Template:Team:KULeuven/12 August 2009/BlueLightReceptor

From 2009.igem.org

(Difference between revisions)
 
(6 intermediate revisions not shown)
Line 1: Line 1:
-
# Gel electrophorese of BlP cut with EcoRI and SpeI
+
# Gel electrophoresis of BlP cut with EcoRI and SpeI
# Gel extraction of the Blp  
# Gel extraction of the Blp  
Nanodrop results
Nanodrop results
Line 8: Line 8:
|-
|-
|}
|}
-
# PCR (with Pfx) of blue light promoter with primers iGEM 2260 and igem 2261
+
# PCR (with Pfx) of blue light promoter with primers iGEM 2260 and iGEM 2261
#* Annealing of 58C
#* Annealing of 58C
-
# Innoculate liquid medium
+
# Inoculate liquid medium
-
#* Innoculation of Psb3k3-P1010 and J23101
+
#* Inoculation of {{kulpart|pSB3K3}}-{{kulpart|BBa_P1010}} and {{kulpart|BBa_J23101}}
# Ligation  
# Ligation  
{| border ="1" align="center"
{| border ="1" align="center"
Line 17: Line 17:
| Vector || insert  
| Vector || insert  
|- align="center"
|- align="center"
-
| {{kulpart|psB1A3}}+P1010 || {{kulpart|BBa_K238013}} (BLP)  
+
| {{kulpart|pSB1A3}}+{{kulpart|BBa_P1010}} || {{kulpart|BBa_K238013}} (BLP)  
|- align="center"
|- align="center"
| 50ng -> 2,5 μl || 5 ng -> 0,3 μl  
| 50ng -> 2,5 μl || 5 ng -> 0,3 μl  
|- align="center"
|- align="center"
|}
|}

Latest revision as of 08:48, 8 September 2009

  1. Gel electrophoresis of BlP cut with EcoRI and SpeI
  2. Gel extraction of the Blp

Nanodrop results

Part concentration (ng/μl) 260/280 λ 260/230 λ
Blp 27 2,08
  1. PCR (with Pfx) of blue light promoter with primers iGEM 2260 and iGEM 2261
    • Annealing of 58C
  2. Inoculate liquid medium
    • Inoculation of - and
  3. Ligation
Vector insert
+ (BLP)
50ng -> 2,5 μl 5 ng -> 0,3 μl