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Template:Team:KULeuven/12 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
| Line 11: | Line 11: | ||
#* Annealing of 58C | #* Annealing of 58C | ||
# Innoculate liquid medium | # Innoculate liquid medium | ||
| - | #* Innoculation of | + | #* Innoculation of {{kulpart|sb3k3}}-{{kulpart|BBa_P1010}} and {{kulpart|BBa_J23101}} |
# Ligation | # Ligation | ||
{| border ="1" align="center" | {| border ="1" align="center" | ||
| Line 17: | Line 17: | ||
| Vector || insert | | Vector || insert | ||
|- align="center" | |- align="center" | ||
| - | | {{kulpart|psB1A3}}+ | + | | {{kulpart|psB1A3}}+{{kulpart|BBa_P1010}} || {{kulpart|BBa_K238013}} (BLP) |
|- align="center" | |- align="center" | ||
| 50ng -> 2,5 μl || 5 ng -> 0,3 μl | | 50ng -> 2,5 μl || 5 ng -> 0,3 μl | ||
|- align="center" | |- align="center" | ||
|} | |} | ||
Revision as of 09:58, 1 September 2009
- Gel electrophorese of BlP cut with EcoRI and SpeI
- Gel extraction of the Blp
Nanodrop results
| Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
|---|---|---|---|
| Blp | 27 | 2,08 |
- PCR (with Pfx) of blue light promoter with primers iGEM 2260 and igem 2261
- Annealing of 58C
- Innoculate liquid medium
- Innoculation of - and
- Ligation
| Vector | insert |
| + | (BLP) |
| 50ng -> 2,5 μl | 5 ng -> 0,3 μl |
