Template:Team:KULeuven/13 August 2009/BlueLightReceptor

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(Difference between revisions)
(New page: # PCR product of 12-august of pairt {{kulpart|BBa_K238013}} # Miniprep of {{kulpart|BBa_J23101}} Nanodrop results: {| border ="1" align="center" !| Part || concentration (ng/μl) || 260...)
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!| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ
!| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ
|- align="center"
|- align="center"
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| {{kulpart|BBa_K238013}} ||123 || 1,82 ||  
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| {{kulpart|BBa_K238013}} ||123,3 || 1,82 ||  
|- align="center"
|- align="center"
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| {{kulpart|BBa_J23101}} ||141|| 1,99 ||   
+
| {{kulpart|BBa_J23101}} ||141,2|| 1,99 ||   
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Revision as of 15:39, 13 August 2009

  1. PCR product of 12-august of pairt
  2. Miniprep of

Nanodrop results:

Part concentration (ng/μl) 260/280 λ 260/230 λ
123,3 1,82
141,2 1,99
  1. Restriction digest cut with EcoRI and XbaI
  2. Electroporation of Psb3k3 + P1010 and the ligation of ...
  3. Gelelectrophorese of
    • Only 1 band, so probably everything was cut
  4. Gelectraction of
    • Nanodrop = 29,3 ng/μl
  5. ligation of vector and insert