Template:Team:KULeuven/14 August 2009/VanillinProduction

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  1. Gel extraction of the 4 lanes from sam8 () and ech ()
    • To test an additional blanc, we extracted a blanc piece of gel
    • Nanodrop results were done with the conventional blanc (just elution buffer) and with the extracted blanco gel. However, since the results between both varied too much and the extracted blanco gel was not significantly better, we decided to use the conventional blanco in order to compare between former nanodrop measurements


Part concentration (ng/μl) 260/280 λ 260/230 λ
sam8 9,4 2,18
ech 13,6 1,94
  • Ligation of sam5 (from Wednesday) + sam8 (extracted today)
vector insert
sam5 sam8
3637 bp 1550 bp
50 ng 85,2 ng
7 μl 9 μl
  • Ligation of ech and fcs. For fcs we took the digest from last week (C1), because this week's restriction digest had a very low concentration (4,2) and a very high 260/280 (6,08)
vector insert
ech fcs
2929 bp 1789 bp
50 ng 122 ng
3 μl 9 μl
  • Cells were stored at 16°C
  • Plated the electroporated cells from the A and C ligation
  • Made Agar and poured new plates with Ap