Template:Team:KULeuven/14 September 2009/VanillinProduction

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Revision as of 13:28, 19 September 2009 by JochemDeen (Talk | contribs)
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  • Miniprep of COMT
part concentration 260/280 260/230
COMT1 1 63,5 1,98
COMT1 2 99,4 1,95
COMT1 3 84,5 1,91
COMT2 1 92,1 2,00
COMT2 2 80,4 1,93
COMT2 3 74,2 1,95
COMT3 1 92,0 2,03
COMT3 2 62,2 2,04
COMT3 3 70,5 2,04
COMT4 1 93,4 1,91
COMT4 2 104,4 1,96
COMT4 3 81,9 2,01
  • Restriction with S/P, SAMS2 P (was already cut with E/X) and EF3 with E/S
  • Another attempt at electroporating EFT using EFT ligation from last week
  • Gel electroforesis of 5 μl from the restriction sample (30 μl)
    • Nothing in EF lane (no DNA???)
    • Promotor showed again two results of 900bp and 2k bp (wut is going on?)
  • An attempt was made to seperate the restriction enzymes by PCR purification on the remaining restriction sample, however nanodrop showed very confusing results, conclusion: let's not try this again. Next time heat inactivation of restriction enzymes to make sure they are inactive.