Template:Team:KULeuven/1 September 2009/VanillinProduction

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(Difference between revisions)
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* SAMS I A RD is put on gel, to be used for gel extraction and a part of SAMS I B RD is put on gel to check the length.
* SAMS I A RD is put on gel, to be used for gel extraction and a part of SAMS I B RD is put on gel to check the length.
** No signal... grrr...
** No signal... grrr...
 +
* Replated colonies from the original SAMS plate from 24/8
 +
** Took 4 individual colonies and plated them on 4 corners of a petri dish

Revision as of 09:00, 2 September 2009

  • SAMS I B RD is used for ligation with TER II RD
    • Since we want to avoid gel purification we use the direct sample from the restriction diget
    • The restriction enzymes are denatured by keeping the sample at 85C for 20 minutes
vector insert
Terminator SAMS
2000 bp 3070 bp
50 ng 280 ng
10 μl 16 μl
  • The genes fcs and ech are individually cut with the four restriction enzymes
EcoR1 fcs RD-E
Spe1 fcs RD-S
Pst1 fcs RD-P
Xba1 fcs RD-X
EcoR1 Ech RD-E
Spe1 Ech RD-S
Pst1 Ech RD-P
Xba1 Ech RD-X
  • Results on gel shows that all the genes have the same length and show 1 line.
  • SAMS I A RD is put on gel, to be used for gel extraction and a part of SAMS I B RD is put on gel to check the length.
    • No signal... grrr...
  • Replated colonies from the original SAMS plate from 24/8
    • Took 4 individual colonies and plated them on 4 corners of a petri dish
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