Template:Team:KULeuven/29 August 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)
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***ech showed 4 results in gel (all too short), expected one
***ech showed 4 results in gel (all too short), expected one
** Sam5 and Sam8 were purified and ligated (Sams I)
** Sam5 and Sam8 were purified and ligated (Sams I)
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{| border ="1" align="center"
{| border ="1" align="center"
!| Part || DNA conc ng/µl|| 260/280
!| Part || DNA conc ng/µl|| 260/280
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|- align="center"
|- align="center"
| Sam8||4,1 || 2,88
| Sam8||4,1 || 2,88
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|- align="center
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|-}
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|}
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* Ech and Fcs were cut again and put overnight (overweekend...) in 16C
* Ech and Fcs were cut again and put overnight (overweekend...) in 16C

Revision as of 10:00, 31 August 2009

  • Split the restriction digest in 2 parts
    • 1 Part was directly used for ligation using double the mass required(Sams II and Ef II)
Part DNA ng volume µl
Sam5100 0,8
Sam8126,4 2,7
Ech 100 1
Fcs 244 2,2
  • The 2nd part was first put on gel electrophorese and purified before it was used for ligation
  • Gel electrophorese of restriction on Sam, Sam8, fcs and ech
    • Sam5 and Sam8 showed expected results
    • fcs and ech was unexpected
      • fcs showed only 1 result on gel, expected two
      • ech showed 4 results in gel (all too short), expected one
    • Sam5 and Sam8 were purified and ligated (Sams I)
Part DNA conc ng/µl 260/280
Sam56,6 1,98
Sam84,1 2,88
  • Ech and Fcs were cut again and put overnight (overweekend...) in 16C
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