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Template:Team:KULeuven/29 August 2009/VanillinProduction
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Revision as of 08:23, 31 August 2009 by JochemDeen (Talk | contribs)
- Split the restriction digest in 2 parts
- 1 Part was directly used for ligation using double the mass required
| Part | DNA ng | volume µl |
|---|---|---|
| Sam5 | 100 | 0,8 |
| Sam8 | 126,4 | 2,7 |
| Ech | 100 | 1 |
| Fcs | 244 | 2,2 |
- 2nd part was first put on gel electrophorese and purified before it was used for ligation
