Template:Team:KULeuven/31 August 2009/VanillinProduction

From 2009.igem.org

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(New page: * Electroporation of SAMS I, SAMS II AND EF II * Restriction digest of ech and fcs from saturday was tested and showed 6 signals with varying lengths for ech and 1 very large signal for fc...)
 
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* Electroporation of SAMS I, SAMS II AND EF II
* Electroporation of SAMS I, SAMS II AND EF II
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* Restriction digest of ech and fcs from saturday was tested and showed 6 signals with varying lengths for ech and 1 very large signal for fcs, both inconsistent. To test for errors in the restriction sites we cut each vector with 1 restriction digest for each of the sites. Giving a total of 4 tests for ech and 4 for fcs.
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* Restriction digest of ''ech'' and ''fcs'' from Saturday was tested and showed 6 signals with varying lengths for ''ech'' and 1 very large signal for ''fcs'', both inconsistent. To test for errors in the restriction sites we cut each vector with 1 restriction digest for each of the sites. Giving a total of 4 tests for'' ech'' and 4 for ''fcs''.
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** Restrction is in 37C overnight.
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** Restriction at 37°C overnight.
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* For ligation of SAMS III RD (from thursday) and TER II we need 44,4 ul of SAMSIII which we dont have thus we cut two additional volumes with SAMSIII overnight
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* For ligation of SAMS III RD (from Thursday) and TER II we need 44,4 µl of SAMS III which we don't have thus we cut two additional volumes with SAMS I overnight
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** SAMS I A RD and SAMS I B RD

Latest revision as of 11:49, 14 September 2009

  • Electroporation of SAMS I, SAMS II AND EF II
  • Restriction digest of ech and fcs from Saturday was tested and showed 6 signals with varying lengths for ech and 1 very large signal for fcs, both inconsistent. To test for errors in the restriction sites we cut each vector with 1 restriction digest for each of the sites. Giving a total of 4 tests for ech and 4 for fcs.
    • Restriction at 37°C overnight.
  • For ligation of SAMS III RD (from Thursday) and TER II we need 44,4 µl of SAMS III which we don't have thus we cut two additional volumes with SAMS I overnight
    • SAMS I A RD and SAMS I B RD