Template:Team:KULeuven/3 August 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)
(New page: We received the ComT, Sam5 and Sam8 from Edinburgh today. They were put in competent cells by electroporation. * Sam5 + RBS in pSB1A2 (with PstI site removed) * Sam8 + RBS in pSB1A2 * Sam...)
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* Sam5 + RBS in pSB1A2 (with PstI site removed)
* Sam5 + RBS in pSB1A2 (with PstI site removed)
* Sam8 + RBS in pSB1A2
* Sam8 + RBS in pSB1A2
-
* Sam8 in pSB1A2 coding sequence {{kulpart|BBa_I742142}}
+
* Sam8 in pSB1A2 coding sequence, {{kulpart|BBa_I742142}}
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* ComT in pSB1A2 coding sequence {{kulpart|BBa_I742107}}
+
* ComT in pSB1A2 coding sequence, {{kulpart|BBa_I742107}}
The cells were put in liquid LB to recuperate from the procedure and transfered onto LB/amp plates and grown overnight at 37°C.
The cells were put in liquid LB to recuperate from the procedure and transfered onto LB/amp plates and grown overnight at 37°C.

Revision as of 08:25, 4 August 2009

We received the ComT, Sam5 and Sam8 from Edinburgh today. They were put in competent cells by electroporation.

  • Sam5 + RBS in pSB1A2 (with PstI site removed)
  • Sam8 + RBS in pSB1A2
  • Sam8 in pSB1A2 coding sequence,
  • ComT in pSB1A2 coding sequence,

The cells were put in liquid LB to recuperate from the procedure and transfered onto LB/amp plates and grown overnight at 37°C.