Template:Team:KULeuven/3 September 2009/VanillinProduction

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  • PCR
    • The PCR from yesterday went very well, we had a huge amount of amplified biobrick DNA of the different parts. Agarose gel electrophoresis was used to test if the different pieces of DNA created by PCR had the correct length.
    • Amplified DNA was purified and cut with different restriction enzymes.
    • After the restriction, Sam5, Sam8 and terminator were ligated in a three-way ligation. The same was done for ech, fcs and terminator. We also started a ligation of just Sam5 and Sam8 and ech and fcs.
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