Template:Team:KULeuven/4 September 2009/BlueLightReceptor

From 2009.igem.org

(Difference between revisions)
Line 4: Line 4:
# A new restriction digest on {{kulpart|BBa_J61002}} and {{kulpart|BBa_K238013}} with EcoRI and SpeI was performed.  
# A new restriction digest on {{kulpart|BBa_J61002}} and {{kulpart|BBa_K238013}} with EcoRI and SpeI was performed.  
# Gel extraction of these new digests. {{kulpart|BBa_J61002}} was nanodropped with a concentration of 28 ng/µl. this was ligated with {{kulpart|BBa_K238013}} (ligX 2)  
# Gel extraction of these new digests. {{kulpart|BBa_J61002}} was nanodropped with a concentration of 28 ng/µl. this was ligated with {{kulpart|BBa_K238013}} (ligX 2)  
-
# Electroporation of pSB3K3
+
# Electroporation of {{kulpart|BBa_pSB3K3}}
   
   
Line 10: Line 10:
#Electroporation of ligX 1&2
#Electroporation of ligX 1&2
-
#There was no growth of cells after electroporation with pSB3K3
+
#There was no growth of cells after electroporation with {{kulpart|BBa_pSB3K3}}
=Sunday=
=Sunday=
# There was good growth of the cells that were electroporated with ligX. 4 single colonies were selected and plated on Ap medium. This was all done in a dark room to avoid activation of the promotor.
# There was good growth of the cells that were electroporated with ligX. 4 single colonies were selected and plated on Ap medium. This was all done in a dark room to avoid activation of the promotor.

Revision as of 14:35, 10 September 2009

  1. Gel extraction performed on the signals from 3/09
    • Nanodrop: 14,7 ng/µl of with a 260/280 of approx 2,12
  2. Ligation with and (ligX 1)
  3. A new restriction digest on and with EcoRI and SpeI was performed.
  4. Gel extraction of these new digests. was nanodropped with a concentration of 28 ng/µl. this was ligated with (ligX 2)
  5. Electroporation of


Saturday

  1. Electroporation of ligX 1&2
  2. There was no growth of cells after electroporation with

Sunday

  1. There was good growth of the cells that were electroporated with ligX. 4 single colonies were selected and plated on Ap medium. This was all done in a dark room to avoid activation of the promotor.