Template:Team:KULeuven/4 September 2009/BlueLightReceptor

From 2009.igem.org

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# Ligation with {{kulpart|BBa_J61002}} and {{kulpart|BBa_K238013}} (ligX 1)  
# Ligation with {{kulpart|BBa_J61002}} and {{kulpart|BBa_K238013}} (ligX 1)  
# A new restriction digest on {{kulpart|BBa_J61002}} and {{kulpart|BBa_K238013}} with EcoRI and SpeI was performed.  
# A new restriction digest on {{kulpart|BBa_J61002}} and {{kulpart|BBa_K238013}} with EcoRI and SpeI was performed.  
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# gel extraction of these new digests. {{kulpart|BBa_J61002}} was nanodropped with a concentration of 28 ng/µl. this was ligated with {{kulpart|BBa_K238013}} (ligX 2)  
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# Gel extraction of these new digests. {{kulpart|BBa_J61002}} was nanodropped with a concentration of 28 ng/µl. this was ligated with {{kulpart|BBa_K238013}} (ligX 2)  
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# electroporatie van pSB3K3
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# Electroporation of {{kulpart|pSB3K3}}
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=saterday=
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#electroporation of ligX 1&2
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#there was no growth of cells after electroporation with pSB3K3
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=sunday=
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# there was good growth of the cells that were electroporated with ligX. 4 single colonies were selected and plated on Ap medium. this was all done in a dark room to avoid activation of the promotor.
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Latest revision as of 10:06, 21 September 2009

  1. Gel extraction performed on the signals from 3/09
    • Nanodrop: 14,7 ng/µl of with a 260/280 of approx 2,12
  2. Ligation with and (ligX 1)
  3. A new restriction digest on and with EcoRI and SpeI was performed.
  4. Gel extraction of these new digests. was nanodropped with a concentration of 28 ng/µl. this was ligated with (ligX 2)
  5. Electroporation of
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