Template:Team:KULeuven/7 August 2009/VanillinReceptor

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  • Primers for mutagenesis have arrived
  • We used the DNA parts from the second PCR to insert them into a TOPO-vector. The second PCR showed more results so that is why we used these fragments.
  • LBmedium was made with X-gal to select cells afterwards.
  • Plasmids were inserted in competent cells by heatshock and those cells were plated out on the X-gal medium.
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