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| html body div.header div.banner { | | html body div.header div.banner { |
| height: 171px; | | height: 171px; |
- | background-image: url(https://static.igem.org/mediawiki/2009/4/42/BannerUlb15.PNG); | + | background-image: url(https://static.igem.org/mediawiki/2009/0/0a/Logoulb1.png); |
| background-repeat: no-repeat; | | background-repeat: no-repeat; |
| } | | } |
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| html body div.footer { | | html body div.footer { |
- | }
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- |
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- | table {
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- | border-style: solid;
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- | border-color: grey;
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- | border-width: 1px;
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- | background-color: #BBBBBB;
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- | cellspacing: 1px;
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- | text-align: center;
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- | }
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- |
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- | tr {
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- | border-style: solid;
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- | border-width: 1px;
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- | background-color: white;
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- | }
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- |
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- | td {
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- | min-width: 60px;
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| } | | } |
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| <li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li> | | <li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li> |
| <li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li> | | <li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li> |
- | <li><a class="mainlink" href="/Team:ULB-Brussels/Modeling">Modeling</a></li> | + | <li><a class="mainlink" href="/Team:ULB-Brussels/Safety">Safety</a></li> |
| <li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li> | | <li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li> |
| <li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li> | | <li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li> |
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| </body> | | </body> |
| </html> | | </html> |
| + | |
| + | |
| + | <html> |
| + | <style type="text/css"> |
| + | |
| + | |
| + | |
| + | table.calendar { margin: 0; padding: 10px; } |
| + | table.calendar td { margin: 0; padding: 2px; vertical-align: top; } |
| + | table.month .heading td { padding:2px; background-color:#3E6DA4; color:#FFFFFF; text-align:center; font-size:120%; font-weight:bold; } |
| + | table.month .dow td { color:#aaa; text-align:center; font-size:110%; } |
| + | table.month td.today { background-color:#ddd; } |
| + | table.month td { |
| + | border: none; |
| + | margin: 0; |
| + | padding: 1pt 1.5pt; |
| + | font-weight: bold; |
| + | font-size: 12pt; |
| + | text-align: right; |
| + | background-color: #eee; |
| + | } |
| + | #bodyContent table.month a { background:none; padding:0 } |
| + | .day-active { color:blue } |
| + | .day-empty { color:grey } |
| + | |
| + | |
| + | </style> |
| + | </html> |
| + | |
| + | {| align="right" |
| + | {| style="color:#0c6;background-color:#FFFFFF;" cellpadding="3" cellspacing="1" border="1" bordercolor="#035e7b" align="right" |
| + | |
| + | !align="right"|{{#calendar: title=ULB |year=2009 | month=07}} |
| + | |- |
| + | !align="right"|{{#calendar: title=ULB |year=2009 | month=08}} |
| + | |- |
| + | !align="right"|{{#calendar: title=ULB |year=2009 | month=09}} |
| + | |
| + | |} |
| + | |
| Start of construction (ligation) in Standard 10. | | Start of construction (ligation) in Standard 10. |
| | | |
July
|
M | T | W | T | F | S | S
|
|
| [http://2009.igem.org/ULB/1_July_2009 1]
| [http://2009.igem.org/ULB/2_July_2009 2]
| [http://2009.igem.org/wiki/index.php?title=ULB/3_July_2009&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=ULB/4_July_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=ULB/5_July_2009&action=edit 5]
|
[http://2009.igem.org/wiki/index.php?title=ULB/6_July_2009&action=edit 6]
| [http://2009.igem.org/ULB/7_July_2009 7]
| [http://2009.igem.org/wiki/index.php?title=ULB/8_July_2009&action=edit 8]
| [http://2009.igem.org/ULB/9_July_2009 9]
| [http://2009.igem.org/wiki/index.php?title=ULB/10_July_2009&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=ULB/11_July_2009&action=edit 11]
| [http://2009.igem.org/ULB/12_July_2009 12]
|
[http://2009.igem.org/wiki/index.php?title=ULB/13_July_2009&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=ULB/14_July_2009&action=edit 14]
| [http://2009.igem.org/ULB/15_July_2009 15]
| [http://2009.igem.org/wiki/index.php?title=ULB/16_July_2009&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=ULB/17_July_2009&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=ULB/18_July_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=ULB/19_July_2009&action=edit 19]
|
[http://2009.igem.org/ULB/20_July_2009 20]
| [http://2009.igem.org/ULB/21_July_2009 21]
| [http://2009.igem.org/ULB/22_July_2009 22]
| [http://2009.igem.org/ULB/23_July_2009 23]
| [http://2009.igem.org/ULB/24_July_2009 24]
| [http://2009.igem.org/ULB/25_July_2009 25]
| [http://2009.igem.org/wiki/index.php?title=ULB/26_July_2009&action=edit 26]
|
[http://2009.igem.org/wiki/index.php?title=ULB/27_July_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=ULB/28_July_2009&action=edit 28]
| [http://2009.igem.org/ULB/29_July_2009 29]
| [http://2009.igem.org/wiki/index.php?title=ULB/30_July_2009&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=ULB/31_July_2009&action=edit 31]
|
|
August
|
M | T | W | T | F | S | S
|
|
|
|
|
| [http://2009.igem.org/wiki/index.php?title=ULB/1_August_2009&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=ULB/2_August_2009&action=edit 2]
|
[http://2009.igem.org/ULB/3_August_2009 3]
| [http://2009.igem.org/ULB/4_August_2009 4]
| [http://2009.igem.org/wiki/index.php?title=ULB/5_August_2009&action=edit 5]
| [http://2009.igem.org/ULB/6_August_2009 6]
| [http://2009.igem.org/ULB/7_August_2009 7]
| [http://2009.igem.org/wiki/index.php?title=ULB/8_August_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=ULB/9_August_2009&action=edit 9]
|
[http://2009.igem.org/wiki/index.php?title=ULB/10_August_2009&action=edit 10]
| [http://2009.igem.org/ULB/11_August_2009 11]
| [http://2009.igem.org/ULB/12_August_2009 12]
| [http://2009.igem.org/ULB/13_August_2009 13]
| [http://2009.igem.org/ULB/14_August_2009 14]
| [http://2009.igem.org/wiki/index.php?title=ULB/15_August_2009&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=ULB/16_August_2009&action=edit 16]
|
[http://2009.igem.org/ULB/17_August_2009 17]
| [http://2009.igem.org/ULB/18_August_2009 18]
| [http://2009.igem.org/ULB/19_August_2009 19]
| [http://2009.igem.org/ULB/20_August_2009 20]
| [http://2009.igem.org/ULB/21_August_2009 21]
| [http://2009.igem.org/wiki/index.php?title=ULB/22_August_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=ULB/23_August_2009&action=edit 23]
|
[http://2009.igem.org/wiki/index.php?title=ULB/24_August_2009&action=edit 24]
| [http://2009.igem.org/ULB/25_August_2009 25]
| [http://2009.igem.org/ULB/26_August_2009 26]
| [http://2009.igem.org/wiki/index.php?title=ULB/27_August_2009&action=edit 27]
| [http://2009.igem.org/ULB/28_August_2009 28]
| [http://2009.igem.org/wiki/index.php?title=ULB/29_August_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=ULB/30_August_2009&action=edit 30]
|
[http://2009.igem.org/ULB/31_August_2009 31]
|
|
September
|
M | T | W | T | F | S | S
|
| [http://2009.igem.org/ULB/1_September_2009 1]
| [http://2009.igem.org/wiki/index.php?title=ULB/2_September_2009&action=edit 2]
| [http://2009.igem.org/ULB/3_September_2009 3]
| [http://2009.igem.org/ULB/4_September_2009 4]
| [http://2009.igem.org/ULB/5_September_2009 5]
| [http://2009.igem.org/wiki/index.php?title=ULB/6_September_2009&action=edit 6]
|
[http://2009.igem.org/ULB/7_September_2009 7]
| [http://2009.igem.org/wiki/index.php?title=ULB/8_September_2009&action=edit 8]
| [http://2009.igem.org/ULB/9_September_2009 9]
| [http://2009.igem.org/ULB/10_September_2009 10]
| [http://2009.igem.org/ULB/11_September_2009 11]
| [http://2009.igem.org/ULB/12_September_2009 12]
| [http://2009.igem.org/wiki/index.php?title=ULB/13_September_2009&action=edit 13]
|
[http://2009.igem.org/ULB/14_September_2009 14]
| [http://2009.igem.org/wiki/index.php?title=ULB/15_September_2009&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=ULB/16_September_2009&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=ULB/17_September_2009&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=ULB/18_September_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=ULB/19_September_2009&action=edit 19]
| [http://2009.igem.org/wiki/index.php?title=ULB/20_September_2009&action=edit 20]
|
[http://2009.igem.org/wiki/index.php?title=ULB/21_September_2009&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=ULB/22_September_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=ULB/23_September_2009&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=ULB/24_September_2009&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=ULB/25_September_2009&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=ULB/26_September_2009&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=ULB/27_September_2009&action=edit 27]
|
[http://2009.igem.org/wiki/index.php?title=ULB/28_September_2009&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=ULB/29_September_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=ULB/30_September_2009&action=edit 30]
|
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Start of construction (ligation) in Standard 10.
1st building: the promoter (BBa_R0011) and RBS (BBa_B0034)in a plasmid destination which contain the brig CcdB (BBa_P1010) (it induces cell death) and a resistance gene. As our plasmids are resistant to ampicillin, the plasmid is chosen destination pSB1C3 with brick BBa_P1010( resistance to chloramphenicol).
Laboratory manipulation
- Thermoporation of BBa_I51020 (plasmid destination resistant to ampicillin) with thermo-skill bacteria thermocompétentes.
- Digestion of BBa_I51020 with EcoRI and PstI.
Computer part
- Order of primers for amplification ccdA + its weak constitutive promoter (promoter mob, not from E. Coli)
Creating BioBricks (CCDA in the forward and reverse direction).
- Creating BioBricks (CCDA in the forward and reverse direction)
- http://partsregistry.org/Part:BBa_K196000:Design
- http://partsregistry.org/Part:BBa_K196001:Design
|